Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
Carbohydr Polym. 2013 Feb 15;92(2):1752-60. doi: 10.1016/j.carbpol.2012.11.029. Epub 2012 Nov 19.
In this work, a series of cellulose/collagen hydrolysate (RC/CH) films were prepared in NaOH/urea aqueous solution via a simple, low-cost and green pathway. To overcome the disadvantages (brittleness, poor water resistance) of CH as biomaterials, CH was combined with regenerated cellulose (RC) film to construct RC/CH composite materials, leading to vast improvement of the water resistance of CH. Crosslinking with genipin further improved the mechanical properties of the RC/CH films in the wet state. Their structure and properties were characterized by elemental analysis, Fourier transform infra-red (FT-IR) spectra, ultraviolet-visible (UV-vis), scanning electron microscopy (SEM), amino acid analysis, tensile testing, cell adhesion and toxicity tests. The mechanical properties and water resistance of the crosslinked RC/CH films were significantly improved, which made collagen hydrolysate as biomaterial could be used at wet state. Moreover, the RC/CH films exhibited good biocompatibility by proliferation of COS7 cells on the surface, supporting cell adhesion and growth.
在这项工作中，通过一种简单、低成本和绿色的方法，在 NaOH/尿素水溶液中制备了一系列纤维素/胶原水解物（RC/CH）薄膜。为了克服 CH 作为生物材料的缺点（脆性、耐水性差），将 CH 与再生纤维素（RC）薄膜结合，构建 RC/CH 复合材料，极大地提高了 CH 的耐水性。用京尼平交联进一步提高了 RC/CH 薄膜在湿态下的力学性能。通过元素分析、傅里叶变换红外（FT-IR）光谱、紫外-可见（UV-vis）、扫描电子显微镜（SEM）、氨基酸分析、拉伸试验、细胞黏附和毒性试验对其结构和性能进行了表征。交联后的 RC/CH 薄膜的力学性能和耐水性得到了显著提高，这使得胶原水解物作为生物材料可以在湿态下使用。此外，RC/CH 薄膜表现出良好的生物相容性，COS7 细胞在其表面增殖，支持细胞黏附和生长。
