State Key Laboratory of Environment Health (Incubation), Ministry of Education, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Carbohydr Polym. 2013 Feb 15;92(2):1880-6. doi: 10.1016/j.carbpol.2012.11.040. Epub 2012 Nov 23.
Chitosan and pectin/organic rectorite (OREC) were initially deposited on the surface of cellulose acetate electrospun nanofibers by a layer-by-layer (LBL) technique to fabricate scaffolds for bacterial inhibition, and the cytotoxicity of the LBL structured scaffolds was also investigated. A couple of opposite charged material, pectin and OREC, were firstly used to fabricate the intercalated composites. The intercalated structure was determined by selected area electron diffraction. Field-emission scanning electron microscope, X-ray diffraction and X-ray photoelectron spectroscopy were applied for the characterization of LBL structured nanofibrous scaffolds. Antibacterial assay results showed that the diameters of the inhibition zone increased from 7.6 to 15.8 mm for Escherichia coli, as well as from 7.4 to 14.2 mm for Staphylococcus aureus. Finally, human epidermal (EP) cells grew well on the LBL films coating. These novel scaffolds could be an ideal candidate for wound dressings and food packaging.
壳聚糖和果胶/有机累托石（OREC）最初通过层层（LBL）技术沉积在醋酸纤维素电纺纳米纤维的表面上，以制备用于抑制细菌的支架，并研究了 LBL 结构支架的细胞毒性。两种带相反电荷的材料，果胶和 OREC，首先用于制备插层复合材料。通过选区电子衍射确定了插层结构。场发射扫描电子显微镜、X 射线衍射和 X 射线光电子能谱用于 LBL 结构纳米纤维支架的表征。抑菌试验结果表明，大肠杆菌的抑菌圈直径从 7.6 毫米增加到 15.8 毫米，金黄色葡萄球菌的抑菌圈直径从 7.4 毫米增加到 14.2 毫米。最后，LBL 膜涂层上的人表皮（EP）细胞生长良好。这些新型支架可能是伤口敷料和食品包装的理想选择。
