Soil and Environmental Biotechnology Division, National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan.
Chemosphere. 2013 Apr;91(5):663-8. doi: 10.1016/j.chemosphere.2013.01.025. Epub 2013 Feb 8.
Plant-bacteria partnership is a promising approach for remediating soil contaminated with organic pollutants. The colonization and metabolic activity of an inoculated microorganism depend not only on environmental conditions but also on the physiological condition of the applied microorganisms. This study assessed the influence of different inoculum pretreatments on survival, gene abundance and catabolic gene expression of an applied strain (Pantoea sp. strain BTRH79) in the rhizosphere of ryegrass vegetated in diesel contaminated soil. Maximum bacterium survival, gene abundance and expression were observed in the soil inoculated with bacterial cells that had been pregrown on complex medium, and hydrocarbon degradation and genotoxicity reduction were also high in this soil. These findings propose that use of complex media for growing plant inocula may enhance bacterial survival and colonization and subsequently the efficiency of pollutant degradation.
植物-细菌伙伴关系是修复有机污染物污染土壤的一种很有前途的方法。接种微生物的定殖和代谢活性不仅取决于环境条件，还取决于所应用微生物的生理状况。本研究评估了不同接种物预处理对污染柴油土壤中黑麦草根际接种的一株（泛菌属菌株 BTRH79）的生存、基因丰度和代谢基因表达的影响。在接种预先在复杂培养基上生长的细菌细胞的土壤中，观察到细菌的最大存活率、基因丰度和表达，并且在这种土壤中，烃类降解和遗传毒性降低也很高。这些发现表明，使用复杂培养基来培养植物接种物可能会增强细菌的生存和定殖能力，从而提高污染物降解的效率。
