Eskandary S, Tahmourespour A, Hoodaji M, Abdollahi A
Isfahan(Khorasgan) Branch, Islamic Azad University, Arghavanieh Bv, Jey street, Isfahan, Iran.
J Environ Health Sci Eng. 2017 Jun 17;15:12. doi: 10.1186/s40201-017-0274-2. eCollection 2017.
Biological methods of polycyclic aromatic hydrocarbons (PAH) contamination elimination typically involve the transformation of contaminants to non-toxic materials by microorganisms and plants and appear to be the most effective methods available.
In this study, and isolated from oil-contaminated soils were inoculated onto seeds before planting in the pot and 3 weeks after planting by syringe injection into the rhizospheric zone in order to study the elimination of PAHs from 's rhizosphere in the greenhouse. Some physical and chemical properties of the soil, PAH concentrations, seeds germination percentage, root and shoot biomasses of the treated samples were examined.
The results showed that the treated samples inoculated with both bacteria had a significantly higher percentage of seed germination and root and shoot biomass compared to other treatments. The concentration of some PAHs reduced significantly (Pvalue < 0.05) in the rhizosphere of the treated samples inoculated with both bacteria compared to in contaminated soils. Concentrations of some PAHs (eg. Naphthalene, Phenanthrene, Benzo[a]anthracene and Dibenzo[a,h]anthracene) even reached below the detection limit of the method. The PAHs concentrations in the treated samples inoculated with bacteria was decreased significantly (Pvalue < 0.05). Therefore, the results showed the high efficiency of the Festuca and bacterial inoculation in eliminating PAHs from the soil.
According to the results, the partnership of with and isolates displayed positive effect on PAHs dissipation and can be effective cleanup technology with high performance.
多环芳烃（PAH）污染消除的生物学方法通常涉及微生物和植物将污染物转化为无毒物质，这似乎是现有的最有效方法。
在本研究中，从油污土壤中分离出的[细菌名称1]和[细菌名称2]，在盆栽种植前接种到[植物名称]种子上，并在种植3周后通过注射器注射到根际区域，以研究温室中[植物名称]根际PAHs的消除情况。检测了处理后样品的土壤理化性质、PAH浓度、种子发芽率、根和地上部生物量。
结果表明，与其他处理相比，接种两种细菌的处理样品种子发芽率、根和地上部生物量显著更高。与污染土壤相比，接种两种细菌的处理样品根际中一些PAHs的浓度显著降低（P值<0.05）。一些PAHs（如萘、菲、苯并[a]蒽和二苯并[a,h]蒽）的浓度甚至低于该方法的检测限。接种细菌的处理样品中PAHs浓度显著降低（P值<0.05）。因此，结果表明[植物名称]与细菌接种在从土壤中消除PAHs方面具有高效性。
根据结果，[植物名称]与[细菌名称1]和[细菌名称2]菌株的组合对PAHs的消散显示出积极作用，并且可以成为一种高效的清理技术。
