Int J Phytoremediation. 2014;16(7-12):1268-77. doi: 10.1080/15226514.2013.828013.
Inoculation of plants with pollutant-degrading and plant growth-promoting microorganisms is a simple strategy to enhance phytoremediation activity. The objective of this study was to determine the effect of inoculation of different bacterial strains, possessing alkane-degradation and 1-amino-cyclopropane-1 -carboxylic acid (ACC) deaminase activity, on plant growth and phytoremediation activity. Carpet grass (Axonopus affinis) was planted in soil spiked with diesel (1% w/w) for 90 days and inoculated with different bacterial strains, Pseudomonas sp. ITRH25, Pantoea sp. BTRH79 and Burkholderia sp. PsJN, individually and in combination. Generally, bacterial application increased total numbers of culturable hydrocarbon-degrading bacteria in the rhizosphere ofcarpet grass, plant biomass production, hydrocarbon degradation and reduced genotoxicity. Bacterial strains possessing different beneficial traits affect plant growth and phytoremediation activity in different ways. Maximum bacterial population, plant biomass production and hydrocarbon degradation were achieved when carpet grass was inoculated with a consortium of three strains. Enhanced plant biomass production and hydrocarbon degradation were associated with increased numbers of culturable hydrocarbon-degrading bacteria in the rhizosphere of carpet grass. The present study revealed that the combined use of different bacterial strains, exhibiting different beneficial traits, is a highly effective strategy to improve plant growth and phytoremediation activity.
接种具有降解污染物和促进植物生长的微生物是增强植物修复活性的一种简单策略。本研究的目的是确定接种具有烷烃降解和 1-氨基环丙烷-1-羧酸（ACC）脱氨酶活性的不同细菌菌株对植物生长和植物修复活性的影响。地毯草（Axonopus affinis）种植在添加了柴油（1%w/w）的土壤中 90 天，并单独或组合接种不同的细菌菌株，如假单胞菌 ITRH25、泛菌 BTRH79 和伯克霍尔德菌 PsJN。通常，细菌的应用增加了地毯草根际可培养烃降解菌的总数，促进了植物生物量的产生，降解了烃类物质，并降低了遗传毒性。具有不同有益特性的细菌菌株以不同的方式影响植物的生长和植物修复活性。当地毯草接种三种菌株的混合物时，可获得最大的细菌种群、植物生物量生产和烃类降解。增强的植物生物量生产和烃类降解与地毯草根际可培养烃降解菌数量的增加有关。本研究表明，联合使用具有不同有益特性的不同细菌菌株是提高植物生长和植物修复活性的一种非常有效的策略。
