Kang E Y, Coleman R D, Pownall H J, Gotto A M, Yang C Y
Department of Medicine, Baylor College of Medicine, Houston, Texas.
J Protein Chem. 1990 Feb;9(1):31-5. doi: 10.1007/BF01024981.
A procedure for rapid and sensitive analysis of carbohydrate in glycoproteins is described. After methanolysis and benzoylation of the monosaccharides and carbohydrates of a glycoprotein, the derivatized sugars were analyzed by reverse-phase high-performance chromatography using a Vydac C18 stationary phase and a mobile phase composed of a water/acetonitrile gradient. The advantages of this procedure over previously described methods are (1) the simple binary solvent system which is used requires no buffering salts and (2) separate sets of peaks from individual sugars obviate the usual need to reacetylate sugar amino groups.
本文描述了一种对糖蛋白中碳水化合物进行快速灵敏分析的方法。在对糖蛋白中的单糖和碳水化合物进行甲醇解和苯甲酰化后，使用Vydac C18固定相和由水/乙腈梯度组成的流动相，通过反相高效液相色谱对衍生化的糖进行分析。该方法相对于先前描述的方法的优点在于：（1）所使用的简单二元溶剂系统不需要缓冲盐；（2）来自各个糖的峰组分离避免了通常对糖氨基进行重新乙酰化的需要。
