高效液相色谱法分析糖蛋白碳水化合物组成的灵敏方法。
Sensitive method for carbohydrate composition analysis of glycoproteins by high-performance liquid chromatography.
作者信息
Takeuchi M, Takasaki S, Inoue N, Kobata A
机构信息
Department of Biochemistry, University of Tokyo, Japan.
出版信息
J Chromatogr. 1987 Jul 29;400:207-13. doi: 10.1016/s0021-9673(01)81613-7.
PMID:3667747
Abstract
A new high-performance liquid chromatographic system has been developed for separating completely the neutral alditols and aminoalditols derived from sugars in complex carbohydrates. In combination with reduction by NaB3H4, this system affords a very sensitive and rapid method of monosaccharide composition analysis of glycoproteins and glycolipids.
摘要
已开发出一种新型高效液相色谱系统，用于完全分离复杂碳水化合物中糖类衍生的中性糖醇和氨基糖醇。结合硼氢化钠还原法，该系统为糖蛋白和糖脂的单糖组成分析提供了一种非常灵敏且快速的方法。
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