Division of Gastroenterology, Mackay Memorial Hospital, Taipei, Taiwan.
Clin Transl Oncol. 2013 Oct;15(10):855-60. doi: 10.1007/s12094-013-1004-6. Epub 2013 Feb 12.
This research aimed to demonstrate the correlation of circulating endothelial cells (CECs) count and serum cytokine levels with side effects and prognosis in rectal cancer patients receiving adjuvant chemoradiation.
Eleven patients received proctectomy, chemoradiotherapy and follow-up for 4 years. Fifty-five blood samples were taken before radiation and during the course. The quantities of CECs were estimated by flow cytometry, and serological factors were measured by ELISA.
The CEC level in patients without tumor recurrence was significantly lower than in patients with tumor recurrence (p < 0.01). The IL-6 and TGF-β1 levels exhibited a similar profile (p < 0.01). For morbidity, the mean CEC level in patients with grade 3 diarrhea was significantly greater than patients with grades 1 (p < 0.001) and 2 diarrhea (p < 0.005).
Levels of CECs, serum IL-6, TGF-β1 and TNF-α during post-operative chemoradiation in rectal cancer patients might be candidate biomarkers for prognosis and morbidity (NCT00325871).
本研究旨在展示循环内皮细胞（CECs）计数和血清细胞因子水平与接受辅助放化疗的直肠癌患者副作用和预后的相关性。
11 名患者接受了直肠切除术、放化疗，并随访了 4 年。在放疗前和放疗过程中采集了 55 份血样。通过流式细胞术估计 CECs 的数量，并通过 ELISA 测量血清因子。
无肿瘤复发患者的 CEC 水平明显低于肿瘤复发患者（p < 0.01）。IL-6 和 TGF-β1 水平呈现相似的模式（p < 0.01）。对于发病率，3 级腹泻患者的平均 CEC 水平明显高于 1 级（p < 0.001）和 2 级腹泻患者（p < 0.005）。
直肠癌患者术后放化疗过程中 CECs、血清 IL-6、TGF-β1 和 TNF-α 水平可能是预后和发病率的候选生物标志物（NCT00325871）。
