Department of Chemistry, East China Normal University, Shanghai, PR China.
Electrophoresis. 2013 Apr;34(8):1241-8. doi: 10.1002/elps.201200320. Epub 2013 Mar 15.
A hollow fiber-based liquid-phase microextraction method has been developed for enrichment of trace chloroanilines in water samples. Target analytes including aniline, three mono-chlorinated aniline isomers (o-chloroaniline, m-chloroaniline, and p-chloroaniline) and four mono-chlorinated methylaniline isomers (2-chloro-4-methylaniline, 3-chloro-4-methylaniline, 4-chloro-2-methylaniline, and 5-chloro-2-methylaniline) were determined by CE with amperometric detection after microextraction. Several factors that affect separation, detection, and extraction efficiency were investigated. Under the optimum conditions, eight aniline compounds could be well separated from other components coexisting in water samples within 25 min, exhibiting a linear calibration over three orders of magnitude (r > 0.998); the obtained enrichment factors were between 51 and 239, and the LODs were in the range of 0.01-0.1 ng/mL. The proposed method has been applied for the analyses of real environmental water and sewage samples with relative recoveries in the range of 83-108%.
一种基于中空纤维的液相微萃取方法已被开发用于水样中痕量氯苯胺的富集。目标分析物包括苯胺、三种一氯取代苯胺异构体（邻氯苯胺、间氯苯胺和对氯苯胺）和四种一氯取代甲苯胺异构体（2-氯-4-甲苯胺、3-氯-4-甲苯胺、4-氯-2-甲苯胺和 5-氯-2-甲苯胺），经微萃取后，采用 CE 安培检测法进行测定。考察了影响分离、检测和萃取效率的几个因素。在最佳条件下，八种苯胺化合物可在 25 分钟内与水样中其他共存成分良好分离，在三个数量级范围内具有良好的线性校准（r > 0.998）；获得的富集因子在 51 到 239 之间，LOD 在 0.01 到 0.1 ng/mL 范围内。该方法已应用于实际环境水样和污水样的分析，相对回收率在 83-108%范围内。
