Chen Fang, Wang Chunqiu, Zhang Meijiang, Zhang Xiaoli, Liu Yan, Ye Jiannong, Chu Qingcui
Department of Chemistry, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, School of Sports and Health Care, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China.
Talanta. 2014 Feb;119:83-9. doi: 10.1016/j.talanta.2013.10.052. Epub 2013 Oct 30.
Hexanal (Hex) and heptanal (Hep) in human blood have been regarded as potential biomarkers of lung cancer. In this work, a hollow-fiber liquid-phase microextraction (HF-LPME) method has been developed for the preconcentration of these trace aldehydes in urine samples. After derivatization with an electroactive compound 2-thiobarbituric acid, these two non-electroactive aldehydes were converted to electroactive adducts, therefore detectable by capillary zone electrophoresis with amperometric detection (CZE-AD) approach. Experimental conditions of derivatization, extraction, electrophoretic separation and detection were optimized. Under the optimum conditions, the enrichment factors for Hex and Hep could reach 320 and 355, respectively. The limits of detection for Hex and Hep were 2.7 and 0.97 nM, respectively; the average recoveries were in the range of 61-95% and relative standard deviation (RSD) values less than 8.5%. The present method has been applied to quantitative analysis of two biomarkers in human urine in lieu of blood samples, and the assay results showed that the contents of Hex (0.99-6.7 μM) and Hep (2.5-6.4 μM) found in the urine sample of the lung cancer patients were significantly higher than those in the healthy volunteers, liver cancer patients, as well as diabetics. The proposed HF-LPME/CZE-AD method may provide a potential alternative for early non-invasive diagnosis of lung cancer disease.
人体血液中的己醛（Hex）和庚醛（Hep）被视为肺癌的潜在生物标志物。在本研究中，开发了一种中空纤维液相微萃取（HF-LPME）方法，用于尿液样本中这些痕量醛类的预富集。在用具有电活性的化合物2-硫代巴比妥酸进行衍生化后，这两种非电活性醛转化为电活性加合物，因此可通过毛细管区带电泳-安培检测（CZE-AD）方法进行检测。对衍生化、萃取、电泳分离和检测的实验条件进行了优化。在最佳条件下，Hex和Hep的富集因子分别可达320和355。Hex和Hep的检测限分别为2.7和0.97 nM；平均回收率在61-95%范围内，相对标准偏差（RSD）值小于8.5%。本方法已用于替代血液样本对人尿液中的两种生物标志物进行定量分析，检测结果表明，肺癌患者尿液样本中Hex（0.99-6.7 μM）和Hep（2.5-6.4 μM）的含量显著高于健康志愿者、肝癌患者以及糖尿病患者。所提出的HF-LPME/CZE-AD方法可能为肺癌疾病的早期非侵入性诊断提供一种潜在的替代方法。
