Departamento de Biología del Desarrollo, San Miguel de Tucumán, Argentina.
Dev Dyn. 2013 May;242(5):572-9. doi: 10.1002/dvdy.23945. Epub 2013 Mar 27.
The axial skeleton develops from the sclerotome, a mesenchymal cell population derived from somites. Sclerotomal cells migrate from somites to the perinotochordal and perineural space where they differentiate into chondrocytes to form cartilage and bone. In anurans, little is known about the way how the sclerotome changes as development proceeds and how these events are regulated at the molecular level. Pax1, Pax9, and Uncx4.1 genes play a central role in the morphogenesis of the axial skeleton in vertebrates, regulating cell proliferation and chondrogenic specification of the sclerotome.
In this work, we cloned and examined through whole-mount in situ hybridization and reverse transcriptase-polymerase chain reaction the expression patterns of pax1, pax9, and uncx transcription factors in the anuran Xenopus laevis.
We found that these genes are similarly expressed in the sclerotome and in the pharyngeal pouch. A detailed analysis of the location of these transcripts showed that they are expressed in different subdomains of the sclerotomal compartment and differ from that observed in other vertebrates.
轴骨骼由体节衍生的中胚层细胞群体 - 体节细胞发育而来。体节细胞从体节迁移到围脊索和围神经空间，在那里分化为软骨细胞形成软骨和骨骼。在无尾两栖动物中，对于体节随着发育进展而发生的变化方式以及这些事件如何在分子水平上被调控知之甚少。 Pax1、Pax9 和 Uncx4.1 基因在脊椎动物的轴骨骼形态发生中发挥核心作用，调控体节的细胞增殖和软骨生成特异性。
在这项工作中，我们通过全胚胎原位杂交和逆转录聚合酶链反应克隆并检测了无尾两栖动物非洲爪蟾 Xenopus laevis 中 pax1、pax9 和 uncx 转录因子的表达模式。
我们发现这些基因在体节和咽囊中相似表达。对这些转录本位置的详细分析表明，它们在体节隔室的不同亚区表达，与在其他脊椎动物中观察到的不同。
