Department of Surgery, School of Veterinary Medicine and Animal Science, University of São Paulo, Brazil.
Microsc Res Tech. 2013 Apr;76(4):381-7. doi: 10.1002/jemt.22177. Epub 2013 Feb 11.
To check if ovariectomy produces alterations in the articular cartilage structure of the tibial proximal epiphysis and if aerobic physical exercise has any influence on these alterations.
Six-month-old female Wistar rats were distributed in three groups: Control group (CG), without ovariectomy and physical activity; Oophorectomy and sedentary group (OSG), with bilateral ovariectomy and without physical activity, and Oophorectomy and trained group (OTG), with bilateral ovariectomy and physical activity during 3 months. All rats were euthanised at 9 months of age. The results were obtained by using histological sections stained with Hematoxylin and Eosin, and Picrosirius stain.
In relation to the thickness of the articular cartilage an increase was observed in the superficial layer in OSG in relation to OTG. The number of chondrocytes did not show any significant difference. The nuclear volume of chondrocytes in the superficial layer was higher in CG than in OSG . In the medium layer, the volume of chondrocytes was higher in CG than in OSG and the OTG. The same occurred when the values of the epiphysial cartilage was considered as a whole. For the collagen, the values of volume density of the collagen fibers were higher in the OSG and in the OTG than the CG. The same occurred with the OTG in relation to OSG.
The depression of estrogen levels produced important changes in the tibial cartilage of Wistar rats mainly on the superficial layer and physical activity seemed to relieve these effects.
检查卵巢切除术是否会改变胫骨近端骺软骨结构，以及有氧运动是否会对这些变化产生影响。
将 6 月龄雌性 Wistar 大鼠分为 3 组：对照组（CG），未行卵巢切除术和体力活动；卵巢切除术和安静组（OSG），行双侧卵巢切除术且无体力活动；卵巢切除术和训练组（OTG），行双侧卵巢切除术且 3 个月体力活动。所有大鼠均在 9 月龄处死。结果采用苏木精-伊红染色和苦味酸天狼星红染色的组织学切片获得。
与 OTG 相比，OSG 中关节软骨浅层厚度增加。软骨细胞数量无显著差异。与 OSG 相比，CG 中浅层软骨细胞的核体积更大。在中层中，CG 中的软骨细胞体积大于 OSG 和 OTG。将骺软骨整体作为一个整体来看也是如此。对于胶原，OSG 和 OTG 中的胶原纤维体积密度值高于 CG。与 OSG 相比，OTG 也是如此。
雌激素水平降低对 Wistar 大鼠胫骨软骨造成了重要影响，主要发生在浅层，而体力活动似乎缓解了这些影响。
