利用碳检测核磁共振光谱探究精氨酸侧链及其动力学：在高pH值下应用于42 kDa人组蛋白去乙酰化酶8 - Suppr

Suppr

超能文献

Probing arginine side-chains and their dynamics with carbon-detected NMR spectroscopy: application to the 42 kDa human histone deacetylase 8 at high pH.

作者信息

Werbeck Nicolas D, Kirkpatrick John, Hansen D Flemming

机构信息

Institute of Structural and Molecular Biology, University College London, Gower Street, WC1E 6BT, London, UK.

出版信息

Angew Chem Int Ed Engl. 2013 Mar 11;52(11):3145-7. doi: 10.1002/anie.201209385. Epub 2013 Feb 10.

DOI:10.1002/anie.201209385

PMID:23401322

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4016738/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2198/4016738/307aa71f972d/anie0052-3145-f1.jpg

相似文献

Probing arginine side-chains and their dynamics with carbon-detected NMR spectroscopy: application to the 42 kDa human histone deacetylase 8 at high pH.利用碳检测核磁共振光谱探究精氨酸侧链及其动力学：在高pH值下应用于42 kDa人组蛋白去乙酰化酶8

Angew Chem Int Ed Engl. 2013 Mar 11;52(11):3145-7. doi: 10.1002/anie.201209385. Epub 2013 Feb 10.

NMR detection of side chain-side chain hydrogen bonding interactions in 13C/15N-labeled proteins.核磁共振检测13C/15N标记蛋白质中侧链-侧链氢键相互作用

J Biomol NMR. 2000 Aug;17(4):305-10. doi: 10.1023/a:1008390813387.

On the function of the internal cavity of histone deacetylase protein 8: R37 is a crucial residue for catalysis.组蛋白去乙酰化酶蛋白 8 的内腔功能：R37 是催化的关键残基。

Bioorg Med Chem Lett. 2011 Apr 1;21(7):2129-32. doi: 10.1016/j.bmcl.2011.01.128. Epub 2011 Feb 2.

Dynamics of the DNA binding domain of the fructose repressor from the analysis of linear correlations between the 15N-1H bond spectral densities obtained by nuclear magnetic resonance spectroscopy.通过核磁共振光谱获得的15N-1H键谱密度之间的线性相关性分析果糖阻遏物DNA结合结构域的动力学

Biochemistry. 1998 Apr 14;37(15):5060-73. doi: 10.1021/bi971706h.

A miniaturized readout strategy for endogenous histone deacetylase activity.一种用于内源性组蛋白脱乙酰酶活性的小型化读出策略。

Mol Biosyst. 2015 Jul;11(7):1820-3. doi: 10.1039/c5mb00326a.

Substrate and inhibitor specificity of class 1 and class 2 histone deacetylases.1类和2类组蛋白去乙酰化酶的底物和抑制剂特异性

J Biotechnol. 2006 Jun 25;124(1):258-70. doi: 10.1016/j.jbiotec.2006.01.030. Epub 2006 Mar 29.

Residue-specific pKa determination of lysine and arginine side chains by indirect 15N and 13C NMR spectroscopy: application to apo calmodulin.通过间接15N和13C NMR光谱法对赖氨酸和精氨酸侧链进行残基特异性pKa测定：应用于脱钙调蛋白

J Am Chem Soc. 2007 Dec 26;129(51):15805-13. doi: 10.1021/ja0721824. Epub 2007 Nov 29.

Structure of the 30-kDa Sin3-associated protein (SAP30) in complex with the mammalian Sin3A corepressor and its role in nucleic acid binding.与哺乳动物 Sin3A 核心抑制物复合物的 30kDa Sin3 相关蛋白 (SAP30) 的结构及其在核酸结合中的作用。

J Biol Chem. 2011 Aug 5;286(31):27814-24. doi: 10.1074/jbc.M111.252494. Epub 2011 Jun 15.

NMR dynamics investigation of ligand-induced changes of main and side-chain arginine N-H's in human phosphomevalonate kinase.人磷酸甲羟戊酸激酶中配体诱导的主链和侧链精氨酸 N-H 变化的 NMR 动力学研究。

J Am Chem Soc. 2010 Feb 24;132(7):2102-3. doi: 10.1021/ja906244j.

Solution structure of a novel zinc finger motif in the SAP30 polypeptide of the Sin3 corepressor complex and its potential role in nucleic acid recognition.Sin3共抑制复合物的SAP30多肽中一种新型锌指基序的溶液结构及其在核酸识别中的潜在作用。

Nucleic Acids Res. 2009 Apr;37(7):2142-52. doi: 10.1093/nar/gkp051. Epub 2009 Feb 17.

引用本文的文献

Synthesis of Selectively C/H/N- Labeled Arginine to Probe Protein Conformation and Interaction by NMR Spectroscopy.用于通过核磁共振光谱法探测蛋白质构象和相互作用的选择性C/H/N标记精氨酸的合成

Chemistry. 2025 Apr;31(24):e202500408. doi: 10.1002/chem.202500408. Epub 2025 Apr 3.

A combined NMR and deep neural network approach for enhancing the spectral resolution of aromatic side chains in proteins.一种结合核磁共振和深度神经网络的方法，用于提高蛋白质中芳香族侧链的光谱分辨率。

Sci Adv. 2024 Dec 20;10(51):eadr2155. doi: 10.1126/sciadv.adr2155.

Parsing Dynamics of Protein Backbone NH and Side-Chain Methyl Groups using Molecular Dynamics Simulations.使用分子动力学模拟解析蛋白质骨架 NH 和侧链甲基基团的动态。

J Chem Theory Comput. 2024 Jul 23;20(14):6316-6327. doi: 10.1021/acs.jctc.4c00378. Epub 2024 Jul 3.

Observation of Arginine Side-Chain Motions Coupled to the Global Conformational Exchange Process in Deubiquitinase A.去泛素化酶A中与全局构象交换过程耦合的精氨酸侧链运动观察

ACS Omega. 2022 Mar 8;7(11):9936-9943. doi: 10.1021/acsomega.2c00492. eCollection 2022 Mar 22.

C Direct Detected NMR for Challenging Systems.C 直接检测 NMR 用于具有挑战性的体系。

Chem Rev. 2022 May 25;122(10):9468-9496. doi: 10.1021/acs.chemrev.1c00871. Epub 2022 Jan 13.

Simultaneous measurement of H-R's for rapid acquisition of backbone and sidechain paramagnetic relaxation enhancements (PREs) in proteins.同时测量 H-R 的方法，可在蛋白质中快速获取骨干和侧链顺磁弛豫增强（PREs）的参数。

J Biomol NMR. 2021 Mar;75(2-3):109-118. doi: 10.1007/s10858-021-00359-9. Epub 2021 Feb 24.

A distal regulatory region of a class I human histone deacetylase.I 类人组蛋白去乙酰化酶的远端调控区。

Nat Commun. 2020 Jul 31;11(1):3841. doi: 10.1038/s41467-020-17610-w.

Racemic phosphorothioate as a tool for NMR investigations of protein-DNA complexes.外消旋硫代磷酸酯作为用于蛋白质 - DNA 复合物核磁共振研究的工具。

J Biomol NMR. 2020 Sep;74(8-9):421-429. doi: 10.1007/s10858-020-00333-x. Epub 2020 Jul 18.

Multiquantum Chemical Exchange Saturation Transfer NMR to Quantify Symmetrical Exchange: Application to Rotational Dynamics of the Guanidinium Group in Arginine Side Chains.多量子化学交换饱和转移 NMR 定量对称交换：在精氨酸侧链胍基旋转动力学中的应用。

J Phys Chem Lett. 2020 Jul 16;11(14):5649-5654. doi: 10.1021/acs.jpclett.0c01322. Epub 2020 Jul 2.

Mass spectrometry assisted arginine side chains assignment of NMR resonances in natural abundance proteins.质谱辅助的天然丰度蛋白质中 NMR 共振的精氨酸侧链分配。

J Biomol NMR. 2020 Mar;74(2-3):173-181. doi: 10.1007/s10858-020-00302-4. Epub 2020 Feb 1.

本文引用的文献

Arginine residues at internal positions in a protein are always charged.蛋白质内部位置的精氨酸残基总是带电荷的。

Proc Natl Acad Sci U S A. 2011 Nov 22;108(47):18954-9. doi: 10.1073/pnas.1104808108. Epub 2011 Nov 11.

On the function of the internal cavity of histone deacetylase protein 8: R37 is a crucial residue for catalysis.组蛋白去乙酰化酶蛋白 8 的内腔功能：R37 是催化的关键残基。

Bioorg Med Chem Lett. 2011 Apr 1;21(7):2129-32. doi: 10.1016/j.bmcl.2011.01.128. Epub 2011 Feb 2.

Activation and inhibition of histone deacetylase 8 by monovalent cations.单价阳离子对组蛋白去乙酰化酶 8 的激活和抑制。

J Biol Chem. 2010 Feb 26;285(9):6036-43. doi: 10.1074/jbc.M109.033399. Epub 2009 Dec 22.

Protein side-chain dynamics and residual conformational entropy.蛋白质侧链动力学与残余构象熵

J Am Chem Soc. 2009 Jan 21;131(2):615-22. doi: 10.1021/ja806475k.

Multiple-timescale dynamics of side-chain carboxyl and carbonyl groups in proteins by 13C nuclear spin relaxation.通过13C核自旋弛豫研究蛋白质侧链羧基和羰基的多时间尺度动力学

J Am Chem Soc. 2008 Nov 26;130(47):15805-7. doi: 10.1021/ja803794g.

Recognition dynamics up to microseconds revealed from an RDC-derived ubiquitin ensemble in solution.溶液中由剩余偶极距耦合（RDC）得出的泛素系综揭示了直至微秒级的识别动力学。

Science. 2008 Jun 13;320(5882):1471-5. doi: 10.1126/science.1157092.

J Am Chem Soc. 2007 Dec 26;129(51):15805-13. doi: 10.1021/ja0721824. Epub 2007 Nov 29.

Structural basis for signal-sequence recognition by the translocase motor SecA as determined by NMR.通过核磁共振确定的转运体马达SecA对信号序列识别的结构基础。

Cell. 2007 Nov 16;131(4):756-69. doi: 10.1016/j.cell.2007.09.039.

An exchange-free measure of 15N transverse relaxation: an NMR spectroscopy application to the study of a folding intermediate with pervasive chemical exchange.一种无交换的15N横向弛豫测量方法：核磁共振波谱在研究具有普遍化学交换的折叠中间体中的应用。

J Am Chem Soc. 2007 Sep 19;129(37):11468-79. doi: 10.1021/ja072717t. Epub 2007 Aug 28.

Substrate binding to histone deacetylases as shown by the crystal structure of the HDAC8-substrate complex.如HDAC8-底物复合物的晶体结构所示，底物与组蛋白脱乙酰酶的结合。

EMBO Rep. 2007 Sep;8(9):879-84. doi: 10.1038/sj.embor.7401047. Epub 2007 Aug 10.

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验