National Institute of Optics, National Research Council INO-CNR, Largo E. Fermi 6, 50125, Florence, Italy; European Laboratory for Non-linear Spectroscopy LENS, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy.
J Biophotonics. 2014 Jan;7(1-2):86-95. doi: 10.1002/jbio.201200230. Epub 2013 Feb 8.
Two optical fibre-based probes for spectroscopic measurements on human tissues were designed and developed. The two probes combine fluorescence and Raman spectroscopy in a multimodal approach. The fluorescence excitation was provided by two laser diodes emitting in the UV (378 nm) and in the visible (445 nm) range, while a third source in the NIR (785 nm) was used for Raman. The device was tested on freshly excised human skin biopsies clinically diagnosed as malignant melanoma, melanocytic nevus, or healthy skin. Discrimination of lesions based on their fluorescence and Raman spectra showed good correlation with the subsequent histological examination.
设计并开发了两种基于光纤的用于人体组织光谱测量的探头。这两种探头将荧光和拉曼光谱结合在一种多模态方法中。荧光激发由两个激光二极管提供，它们分别在紫外（378nm）和可见（445nm）范围内发射，而近红外（785nm）的第三个光源用于拉曼。该设备在临床上诊断为恶性黑色素瘤、黑素细胞痣或健康皮肤的新鲜切除的人体皮肤活检上进行了测试。基于病变的荧光和拉曼光谱进行区分，与随后的组织学检查具有很好的相关性。
