前沿：新型疫苗接种方式显著预防高致病性猴免疫缺陷病毒的粘膜感染。
Cutting edge: novel vaccination modality provides significant protection against mucosal infection by highly pathogenic simian immunodeficiency virus.
机构信息
Department of Microbiology and Immunology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
出版信息
J Immunol. 2013 Mar 15;190(6):2495-9. doi: 10.4049/jimmunol.1202655. Epub 2013 Feb 11.
Abstract
Vaccine-induced protection against infection by HIV or highly pathogenic and virulent SIV strains has been limited. In a proof-of-concept study, we show that a novel vaccine approach significantly protects rhesus macaques from mucosal infection by the highly pathogenic strain SIVmac251. We vaccinated three cohorts of 12 macaques each with live, irradiated vaccine cells secreting the modified endoplasmic reticulum chaperone gp96-Ig. Cohort 1 was vaccinated with cells secreting gp96(SIV)Ig carrying SIV peptides. In addition, Cohort 2 received recombinant envelope protein SIV-gp120. Cohort 3 was injected with cells secreting gp96-Ig (no SIV Ags) vaccines. Cohort 2 was protected from infection. After seven rectal challenges with highly pathogenic SIVmac251, the hazard ratio was 0.27, corresponding to a highly significant, 73% reduced risk for viral acquisition. The apparent success of the novel vaccine modality recommends further study.
摘要
疫苗诱导对 HIV 或高致病性和高毒力 SIV 株感染的保护作用有限。在一项概念验证研究中，我们表明，一种新型疫苗方法可显著保护恒河猴免受高致病性 SIVmac251 的粘膜感染。我们用分泌修饰的内质网伴侣 gp96-Ig 的活的、辐照的疫苗细胞对三组各 12 只猕猴进行了疫苗接种。第 1 组用携带 SIV 肽的 gp96（SIV）Ig 分泌的细胞进行疫苗接种。此外，第 2 组接受了重组包膜蛋白 SIV-gp120。第 3 组注射了分泌 gp96-Ig（无 SIV Ag）疫苗的细胞。第 2 组免受感染。在接受 7 次具有高致病性的 SIVmac251 的直肠挑战后，危险比为 0.27，这对应于病毒获得风险显著降低 73%。这种新型疫苗模式的明显成功推荐进一步研究。
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