Olegário Janaínna Grazielle Pacheco, Silva Marcos Vinícius, Machado Juliana Reis, Rocha Laura Penna, Reis Marlene Antônia, Guimarães Camila Souza de Oliveira, Corrêa Rosana Rosa Miranda
Discipline of General Pathology, Triângulo Mineiro Federal University, Avenida Frei Paulino No. 30, Bairro Abadia, 38025-180 Uberaba, MG, Brazil.
Clin Dev Immunol. 2013;2013:340959. doi: 10.1155/2013/340959. Epub 2013 Jan 16.
To analyze the cytokines of the innate immune pulmonary response and the capacity for local response to melatonin according to the perinatal stress.
49 cases of pediatric autopsies were evaluated, divided according to cause of death, perinatal stress, gestational age, and birth weight. The percentages of IL-6, C-reactive protein (CRP), IL-1β, TNF-α, and melatonin receptor were evaluated by immunohistochemistry.
The IL-6 expression was higher in the children showing chronic stress, anoxia, and infection. The IL-6 expression showed a progressive increase according to the relation between weight and GA. There was no significant difference in the expression of IL-1β and TNF-α. The CRP expression was higher in the cases showing chronic stress and premature cases. The expression of melatonin receptors was significantly higher in the cases showing chronic stress, being more evident in the cases showing infection.
The cause of death and the type of stress influence the expression in situ of melatonin and cytokines of the innate immune pulmonary response. The evaluation of IL-6 and CRP may contribute to the understanding of the evolution of neonates with chronic stress. The greater sensitivity of the lung to melatonin in these cases may indicate an attempt at controlling the immunological response, in an attempt to diminish the harmful effects of stress.
根据围产期应激情况分析先天性免疫肺部反应的细胞因子以及褪黑素的局部反应能力。
对49例儿科尸检病例进行评估，根据死亡原因、围产期应激、胎龄和出生体重进行分组。通过免疫组织化学评估白细胞介素-6（IL-6）、C反应蛋白（CRP）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和褪黑素受体的百分比。
IL-6表达在显示慢性应激、缺氧和感染的儿童中较高。IL-6表达根据体重与胎龄的关系呈逐渐增加趋势。IL-1β和TNF-α的表达无显著差异。CRP表达在显示慢性应激的病例和早产病例中较高。褪黑素受体的表达在显示慢性应激的病例中显著较高，在显示感染的病例中更明显。
死亡原因和应激类型影响褪黑素和先天性免疫肺部反应细胞因子的原位表达。对IL-6和CRP的评估可能有助于理解患有慢性应激的新生儿的病情发展。在这些病例中，肺部对褪黑素的更高敏感性可能表明试图控制免疫反应，以减轻应激的有害影响。
