从非洲爪蟾XTC细胞条件培养基中分离和鉴定转化生长因子-β2和转化生长因子-β5 。

Isolation and characterization of TGF-beta 2 and TGF-beta 5 from medium conditioned by Xenopus XTC cells.

作者信息

Roberts A B, Rosa F, Roche N S, Coligan J E, Garfield M, Rebbert M L, Kondaiah P, Danielpour D, Kehrl J H, Wahl S M

机构信息

Laboratory of Chemoprevention, National Cancer Institute, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.

出版信息

Growth Factors. 1990;2(2-3):135-47. doi: 10.3109/08977199009071500.

DOI:10.3109/08977199009071500

PMID:2340184

Abstract

TGF-beta 2 and -beta 5 have been purified from medium conditioned by Xenopus cultured cells (XTC) and identified based on their N-terminal amino acid sequence analysis and biological activity. When applied in high concentrations, Xenopus TGF-beta 2, like porcine TGF-beta 2, induces expression of mesodermal markers from cultured Xenopus ectodermal explants, whereas TGF-beta 5 is inactive in this assay. However, the TGF-beta 's could be separated from the major mesoderm-inducing activity present in XTC medium. Xenopus TGF-beta 2 and -beta 5 are approximately equivalent to TGF-beta 1 in their abilities to inhibit the growth of mink lung CCL-64 cells, induce anchorage-independent growth of rat NRK cells, inhibit the proliferation and antibody secretion of human B-lymphocytes, and stimulate chemotaxis of human monocytes. These data establish the functional activity of TGF-beta 5 and suggest that more complex multicellular systems, in contrast to most isolated cells, discriminate between the different TGF-beta s.

摘要

转化生长因子β2（TGF-β2）和β5已从非洲爪蟾培养细胞（XTC）条件培养基中纯化出来，并根据其N端氨基酸序列分析和生物活性进行了鉴定。当高浓度应用时，非洲爪蟾TGF-β2与猪TGF-β2一样，能诱导培养的非洲爪蟾外胚层外植体中中胚层标志物的表达，而TGF-β5在此测定中无活性。然而，这些转化生长因子（TGF-βs）可与XTC培养基中存在的主要中胚层诱导活性分离。非洲爪蟾TGF-β2和β5在抑制貂肺CCL-64细胞生长、诱导大鼠NRK细胞非贴壁依赖性生长、抑制人B淋巴细胞增殖和抗体分泌以及刺激人单核细胞趋化性方面的能力与TGF-β1大致相当。这些数据证实了TGF-β5的功能活性，并表明与大多数分离细胞相比，更复杂的多细胞系统能够区分不同的TGF-βs。