Kothera Linda, Godsey Marvin, Mutebi John-Paul, Savage Harry M
Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Arbovirus Disease Branch, 3150 Rampart Road, Building 401, Fort Collins, CO 80521, USA.
J Am Mosq Control Assoc. 2012 Dec;28(4 Suppl):106-12. doi: 10.2987/8756-971X-28.4.106.
Aboveground and belowground populations of the mosquito Culex pipiens pipiens are traditionally classified as form (f.) pipiens and f. molestus, respectively, and gene flow between forms is thought to be limited. Relatively few f. molestus populations have been found in the United States, which has hindered their study in North America. In this investigation, we used microsatellites to characterize a recently discovered population of f. molestus in Chicago, IL, and compared levels of genetic diversity and differentiation in above-ground and below-ground populations from Chicago and New York City, NY. Levels of genetic diversity were markedly lower in both f. molestus populations. Pairwise F(ST) values between populations indicated that f. molestus populations were highly divergent from each other, as well as from their associated aboveground populations. The most likely number of genetic clusters depended on the number of loci used; we began with a set of 8, and reanalyzed the specimens with 17. Using a panel of 17 loci, there were 4 clusters, 1 for each below-ground population, and 1 for each pair of above-ground populations. Our findings are supportive of the hypothesis that f. molestus populations in Chicago and New York City arose from local aboveground populations.
致倦库蚊的地上和地下种群传统上分别被归类为尖音库蚊指名亚种和骚扰库蚊，并且认为不同类型之间的基因流动是有限的。在美国发现的骚扰库蚊种群相对较少，这阻碍了在北美的相关研究。在本调查中，我们使用微卫星对伊利诺伊州芝加哥市最近发现的骚扰库蚊种群进行特征分析，并比较了芝加哥和纽约市地上和地下种群的遗传多样性和分化水平。两个骚扰库蚊种群的遗传多样性水平均显著较低。种群间的成对F(ST)值表明，骚扰库蚊种群彼此之间以及与其相关的地上种群之间存在高度分化。最可能的基因簇数量取决于所使用的基因座数量；我们从一组8个基因座开始，并使用17个基因座重新分析样本。使用一组17个基因座时，有4个基因簇，每个地下种群1个，每对地上种群1个。我们的研究结果支持了这样的假设，即芝加哥和纽约市的骚扰库蚊种群源自当地的地上种群。
