Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, College of Life Science, South China Normal University, Guangzhou 510631, PR China.
Fish Shellfish Immunol. 2013 May;34(5):1131-8. doi: 10.1016/j.fsi.2013.01.016. Epub 2013 Feb 9.
Five feeding trials based on the isonitrogenous and isoenergetic experimental diets containing 34% protein, 6%, 8%, 10%, 12% or 14% lipid respectively in the circulating water culture system for both 30 and 60 days were conducted to investigate the effect of the dietary lipid level on the growth and immunity in white shirmp, Litopenaeus vannamei adults. The body weight and specific growth rate of white shrimp in different treatments indicated that shrimps fed the diet of 12% lipid level for 30d and 10% lipid level for 60d had the best developmental status. The ability of respiratory burst in hemocytes was improved as the increase of dietary lipid level. The transcripts of LGBP and pPO were sensitive to the dietary lipid in hemocyte and hepatopancreas respectively. The activities of CAT, GPx and AKP were increased to a certain extend according to dietary lipid level. Qualification of MDA showed the lowest level in the sample subjected to 12% lipid level diet, indicating an optimal utilization of the dietary lipid and an efficient clearance of MDA in vivo. These results suggested that dietary lipid level of 10-12% significantly tunes the growth and enhance the immune abilities mainly via ROS pathway of L. vannamei.
在循环水养殖系统中进行了五项基于等氮和等能实验饲料的喂养试验，饲料中分别含有 34%的蛋白质、6%、8%、10%、12%或 14%的脂肪，分别喂养 30 天和 60 天，以研究膳食脂肪水平对南美白对虾成虾生长和免疫力的影响。不同处理组虾的体重和特定生长率表明，摄食 12%脂肪水平饲料 30 天和 10%脂肪水平饲料 60 天的虾具有最佳的发育状态。血细胞呼吸爆发能力随着膳食脂肪水平的增加而提高。LGBP 和 pPO 的转录本在血细胞和肝胰腺中分别对膳食脂肪敏感。根据膳食脂肪水平，CAT、GPx 和 AKP 的活性均有一定程度的增加。MDA 的定性分析表明，12%脂肪水平饮食组的样品 MDA 水平最低，表明体内膳食脂肪的有效利用和 MDA 的有效清除。这些结果表明，10-12%的膳食脂肪水平显著调节南美白对虾的生长，增强免疫能力，主要通过 ROS 途径。
