Department of Clinical Dentistry, Center for Clinical Dental Research, University of Bergen, Norway.
Int J Oral Maxillofac Surg. 2013 Jul;42(7):907-14. doi: 10.1016/j.ijom.2013.01.003. Epub 2013 Feb 9.
Hyperbaric oxygen (HBO) therapy is used to treat or prevent tissue necrosis in patients undergoing irradiation. Many such patients require reconstructive surgery, but little is known of the effects of HBO on bone vascularization and regeneration. In this study, copolymer poly(l-lactide-co-1,5-dioxepan-2-one) (poly(LLA-co-DXO)) scaffolds were implanted into critical-sized calvarial defects in Wistar rats. The animals were randomly allotted to hyperbaric or normobaric oxygen groups. The treatment group received five sessions weekly for 90 min at increased atmospheric pressure, for up to 4 weeks. Samples were retrieved at weeks 2 and 8, i.e. after a total of 10 and 20 sessions, respectively. The samples were analyzed by real-time reverse transcriptase polymerase chain reaction (RT-PCR) and histology at week 2, and radiographically and histologically at week 8. At week 2, defects treated with HBO exhibited greater numbers of cells positive for the endothelial marker CD31, up-regulated gene expression of osteogenic markers, and down-regulated expression of pro-inflammatory cytokines. At week 8, radiographic examination revealed that calvarial defects subjected to HBO exhibited a higher percentage of radiopacities than normobaric controls, and histological examination disclosed enhanced bone healing. These results confirmed that HBO treatment was effective in stimulating vascularization and bone formation in rat calvarial defects.
高压氧（HBO）治疗用于治疗或预防接受照射的患者的组织坏死。许多此类患者需要进行重建手术，但对 HBO 对骨血管化和再生的影响知之甚少。在这项研究中，将共聚物聚（L-丙交酯-共-1,5-二恶烷-2-酮）（聚（LLA-co-DXO））支架植入 Wistar 大鼠的临界尺寸颅骨缺损中。动物被随机分配到高压或常压氧组。治疗组每周接受五次治疗，每次 90 分钟，在增加的大气压下进行，最多持续 4 周。在第 2 和第 8 周分别取出样本，即总共进行了 10 和 20 次治疗后。第 2 周通过实时逆转录聚合酶链反应（RT-PCR）和组织学分析，第 8 周通过放射学和组织学分析进行分析。第 2 周，用 HBO 治疗的缺陷表现出更多的内皮标志物 CD31 阳性细胞，上调成骨标志物的基因表达，并下调促炎细胞因子的表达。第 8 周，放射学检查显示，接受 HBO 的颅骨缺陷比常压对照组表现出更高比例的射线不透性，组织学检查显示骨愈合增强。这些结果证实，HBO 治疗有效刺激了大鼠颅骨缺损的血管生成和骨形成。
