Jan Ahmed M A, Sándor George K B, Iera Deborah, Mhawi Amir, Peel Sean, Evans A Wayne, Clokie Cameron M L
University of Toronto, Toronto, Canada.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 Feb;101(2):144-9. doi: 10.1016/j.tripleo.2005.08.032.
This study was undertaken to evaluate whether the effects of hyperbaric oxygen (HBO) therapy could alter the critical size for spontaneous healing of a bone defect in the rabbit calvarial model.
An animal trial of 12 weeks duration was conducted using 20 New Zealand white rabbits, which were randomly divided into 2 groups of 10 animals each. Calvarial defects were created in the parietal bones of each animal bilaterally. Defects were critical-sized, 15 mm on one side and supra-critical-sized, 18 mm on the contralateral side. Group 1 received a 90-min HBO treatment sessions at 2.4 absolute atmospheric pressure (ATA) per day for 20 consecutive days. Group 2 served as a control without any HBO treatment sessions. Five animals in each group were sacrificed at 6 and 12 weeks. Data analysis included qualitative assessment of the calvarial specimens, post-sacrifice radiographs, as well as histomorphometric analysis to compute the amount of regenerated bone within the defects. ANOVA and paired sample t test were used for statistical analysis.
Both radiographic analysis and histomorphometric analysis demonstrated that HBO-treated animals had significantly more new bone within their defects compared with the control group (P < .001). There was no statistically significant difference between the percentage of new bone forming in the 15-mm and 18-mm HBO-treated defects. There was no difference between the 6-week and the 12-week HBO-treated groups. HBO is effective in enhancing the bony healing of full thickness critical sized as well as supra-critical-sized defects in the rabbit calvarial model.
Bone regeneration was significantly greater in the HBO-treated animals regardless of the defect size. HBO may have increased the diameter of the rabbit critical-sized calvarial defect to more than 18 mm.
本研究旨在评估高压氧（HBO）治疗是否能改变兔颅骨模型中骨缺损自发愈合的临界尺寸。
使用20只新西兰白兔进行了一项为期12周的动物试验，将其随机分为2组，每组10只动物。在每只动物的双侧顶骨上制造颅骨缺损。缺损为临界尺寸，一侧为15毫米，对侧为超临界尺寸，18毫米。第1组每天在2.4绝对大气压（ATA）下接受90分钟的HBO治疗，连续20天。第2组作为对照组，不进行任何HBO治疗。每组5只动物在6周和12周时处死。数据分析包括对颅骨标本的定性评估、处死后人像X线片，以及组织形态计量分析以计算缺损内再生骨的量。采用方差分析和配对样本t检验进行统计分析。
影像学分析和组织形态计量分析均表明，与对照组相比，接受HBO治疗的动物缺损内有明显更多的新骨（P <.001）。在接受HBO治疗的15毫米和18毫米缺损中，新骨形成的百分比之间没有统计学上的显著差异。接受HBO治疗的6周组和12周组之间没有差异。在兔颅骨模型中，HBO能有效促进全层临界尺寸以及超临界尺寸缺损的骨愈合。
无论缺损大小，接受HBO治疗的动物骨再生明显更多。HBO可能使兔临界尺寸颅骨缺损的直径增加到超过18毫米。
