CREM, Department of Life Sciences, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal.
Antimicrob Agents Chemother. 2013 Apr;57(4):2001-2. doi: 10.1128/AAC.02510-12. Epub 2013 Feb 12.
Recently, we have demonstrated that the cognate regulatory locus of the mecA gene in methicillin-resistant Staphylococcus aureus (MRSA) is in fact a three-component system containing the novel mecR2 gene coding for an antirepressor. MecR2 interacts with the repressor MecI, disturbing its binding to the mecA promoter and fostering its proteolysis. Here, we engineered a point mutation in the putative cleavage site of MecI and demonstrated that MecI proteolysis is strictly required for the optimal expression of β-lactam resistance.
最近，我们已经证明了耐甲氧西林金黄色葡萄球菌（MRSA）中 mecA 基因的同源调节基因座实际上是一个三组分系统，包含编码反阻遏物的新型 mecR2 基因。MecR2 与阻遏物 MecI 相互作用，干扰其与 mecA 启动子的结合，并促进其蛋白水解。在这里，我们在假定的 MecI 切割位点上设计了一个点突变，并证明了 MecI 蛋白水解对于β-内酰胺抗性的最佳表达是严格必需的。
