Kawano S, Anderson R W, Nanba T, Kuroiwa T
Department of Cell Biology, National Institute for Basic Biology, Okazaki, Japan.
J Gen Microbiol. 1987 Nov;133(11):3175-82. doi: 10.1099/00221287-133-11-3175.
Restriction endonuclease analysis was done on mitochondrial DNA (mtDNA) from 19 plasmodial strains of Physarum polycephalum. The extent of mtDNA variation among these strains was high in comparison with other organisms, and provides a useful source of cytoplasmic genetic markers. The strains were classified into seven groups according to their mtDNA types. Although plasmodia of P. polycephalum are diploid, formed by fusion of amoebal isogametes, each of the 19 plasmodia possessed mtDNA of only a single type. The transmission pattern of mtDNA during plasmodium formation was studied by mating pairs of amoebal strains that contained mtDNA of different types. Transmission was uniparental; the plasmodia that were formed carried mtDNA with the restriction pattern of only one of the two parental types. Since diploid zygotes develop into plasmodia by repeated mitotic cycles in the absence of cell division, it is clear that this uniparental transmission of mtDNA does not depend upon random partitioning either of mitochondria or of mtDNA molecules during cell division.
对多头绒泡菌的19个疟原虫菌株的线粒体DNA（mtDNA）进行了限制性内切酶分析。与其他生物体相比，这些菌株中mtDNA的变异程度很高，并且提供了有用的细胞质遗传标记来源。根据其mtDNA类型，这些菌株被分为七组。尽管多头绒泡菌的疟原虫是二倍体，由变形虫同型配子融合形成，但19个疟原虫中的每一个都只拥有单一类型的mtDNA。通过交配含有不同类型mtDNA的变形虫菌株对，研究了疟原虫形成过程中mtDNA的传递模式。传递是单亲的；形成的疟原虫携带的mtDNA具有两种亲本类型中仅一种的限制性模式。由于二倍体合子在没有细胞分裂的情况下通过重复的有丝分裂周期发育成疟原虫，很明显，mtDNA的这种单亲传递不依赖于细胞分裂期间线粒体或mtDNA分子的随机分配。
