IRTA , Carretera de Poble Nou, km 5.5, 43540 Sant Carles de la Ràpita, Spain.
J Agric Food Chem. 2013 Mar 13;61(10):2572-9. doi: 10.1021/jf305334n. Epub 2013 Feb 27.
The protein phosphatase inhibition assay (PPIA) is a well-known strategy for the determination of diarrheic shellfish poisoning (DSP) lipophilic toxins, which deserves better characterization and understanding to be used as a routine screening tool in monitoring programs. In this work, the applicability of two PPIAs to the determination of okadaic acid (OA), dinophysistoxin-1 (DTX-1), dinophysistoxin-2 (DTX-2), and their acyl ester derivatives in shellfish has been investigated. The inhibitory potencies of the DSP toxins on a recombinant and a wild PP2A have been determined, allowing the establishment of inhibition equivalency factors (IEFs) (1.1 and 0.9 for DTX-1, and 0.4 and 0.6 for DTX-2, for recombinant and wild PP2A, respectively). The PPIAs have been applied to the determination of OA equivalent contents in spiked and naturally contaminated shellfish samples. Results have been compared to those obtained by LC-MS/MS analysis, after application of the IEFs, showing good agreements.
蛋白磷酸酶抑制分析（PPIA）是一种用于测定腹泻性贝类毒素（DSP）脂溶性毒素的常用策略，为了将其更好地应用为监测计划中的常规筛选工具，值得对其进行更好的特征描述和理解。在这项工作中，研究了两种 PPIA 测定贝类中 okadaic 酸（OA）、dinophysistoxin-1（DTX-1）、dinophysistoxin-2（DTX-2）及其酰基酯衍生物的适用性。测定了 DSP 毒素对重组和野生型 PP2A 的抑制效力，从而确定了抑制等效因子（IEF）（对于重组和野生型 PP2A，DTX-1 分别为 1.1 和 0.9，DTX-2 分别为 0.4 和 0.6）。应用 PPIA 测定了加标和自然污染贝类样品中的 OA 当量含量。应用 IEF 后，将结果与 LC-MS/MS 分析的结果进行了比较，结果吻合良好。
