Safaepour Mona, Shahverdi Ahmad Reza, Shahverdi Hamid Reza, Khorramizadeh Mohammad Reza, Gohari Ahmad Reza
Department of Pharmaceutical Biotechnology and Biotechnology Research center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Avicenna J Med Biotechnol. 2009 Jul;1(2):111-5.
Many reports have been published about the biogenesis of silver nanoparticles using several plant extracts such as Pelargonium graveolens (P.graveolens- geranium) and Azadirachta indica (neem) but the capacity of their natural reducing constituents to form silver nanoparticles has not yet been studied. In this research the synthesis of silver nanoparticles using geraniol has been investigated. We successfully synthesized uniformly dispersed silver nanoparticles with a uniform size and shape in the range of 1 to 10 nm with an average size of 6 nm. Also the cytotoxicity of the prepared silver nanoparticles was investigated using a cancer cell line (Fibrosarcoma-Wehi 164). The cytotoxicity analysis of the sample shows a direct dose-response relationship; cytotoxicity increased at higher concentrations. At concentration of 1 µg/ml, silver nanoparticles was able to inhibit the cell line's growth by less than 30%. Conversly, the presence of 5 µg/ml of silver nanoparticlse significantly inhibited the cell line's growth (>60%). The concentration necessary to produce 50% cell death was 2.6 µg/ml for this silver nanoparticles preapared with geraniol.
已经发表了许多关于使用几种植物提取物（如香叶天竺葵（P.graveolens - 天竺葵）和印楝（印楝树））生物合成银纳米颗粒的报告，但尚未研究其天然还原成分形成银纳米颗粒的能力。在本研究中，对使用香叶醇合成银纳米颗粒进行了研究。我们成功合成了尺寸和形状均匀、尺寸在1至10纳米范围内且平均尺寸为6纳米的均匀分散的银纳米颗粒。此外，使用癌细胞系（纤维肉瘤 - Wehi 164）对制备的银纳米颗粒的细胞毒性进行了研究。样品的细胞毒性分析显示出直接的剂量 - 反应关系；在较高浓度下细胞毒性增加。在1μg/ml的浓度下，银纳米颗粒能够抑制细胞系的生长不到30%。相反，5μg/ml的银纳米颗粒的存在显著抑制了细胞系的生长（>60%）。对于用香叶醇制备的这种银纳米颗粒，产生50%细胞死亡所需的浓度为2.6μg/ml。
