Minhas Lubna Anjum, Kaleem Muhammad, Jabeen Amber, Ullah Nabi, Farooqi Hafiz Muhammad Umer, Kamal Asif, Inam Farooq, Alrefaei Abdulwahed Fahad, Almutairi Mikhlid H, Mumtaz Abdul Samad
Department of Plant Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan.
Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, West Hollywood, Los Angeles, CA 90048, USA.
Microorganisms. 2023 Oct 12;11(10):2544. doi: 10.3390/microorganisms11102544.
Nanoparticles have emerged as a prominent area of research in recent times, and silver nanoparticles (AgNPs) synthesized via phyco-technology have gained significant attention due to their potential therapeutic applications. , a unique and lesser-explored cyanobacterial strain, holds substantial promise as a novel candidate for synthesizing nanoparticles. This noticeable research gap underscores the novelty and untapped potential of in applied nanotechnology. A range of analytical techniques, including UV-vis spectral analysis, dynamic light scattering spectroscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray powder diffraction, were used to investigate and characterize the AgNPs. Successful synthesis of AgNPs was confirmed through UV-visible spectroscopy, which showed a surface plasmon resonance peak at 428 nm. The crystalline size of AgNPs was 24.1 nm. Dynamic light scattering analysis revealed that silver oxide nanoparticles had 179.3 nm diameters and a negative surface charge of -18 mV. Comprehensive in vitro pharmacogenetic properties revealed that AgNPs have significant therapeutic potential. The antimicrobial properties of AgNPs were evaluated by determining the minimum inhibitory concentration against various microbial strains. Dose-dependent cytotoxicity assays were performed on Leishmanial promastigotes (IC: 18.71 μgmL), amastigotes (IC50: 38.6 μgmL), and brine shrimps (IC: 134.1 μg mL) using various concentrations of AgNPs. The findings of this study revealed that AgNPs had significant antioxidant results (DPPH: 57.5%, TRP: 55.4%, TAC: 61%) and enzyme inhibition potential against protein kinase (ZOI: 17.11 mm) and alpha-amylase (25.3%). Furthermore, biocompatibility tests were performed against macrophages (IC: >395 μg mL) and human RBCs (IC: 2124 μg mL). This study showed that phyco-synthesized AgNPs were less toxic and could be used in multiple biological applications, including drug design and in the pharmaceutical and biomedical industries. This study offers valuable insights and paves the way for further advancements in AgNPs research.
近年来，纳米颗粒已成为一个重要的研究领域，通过藻类技术合成的银纳米颗粒（AgNPs）因其潜在的治疗应用而备受关注。 ，一种独特且较少被探索的蓝藻菌株，作为合成纳米颗粒的新型候选者具有巨大潜力。这一明显的研究空白凸显了 在应用纳米技术中的新颖性和未开发的潜力。一系列分析技术，包括紫外可见光谱分析、动态光散射光谱、扫描电子显微镜、傅里叶变换红外光谱和X射线粉末衍射，被用于研究和表征AgNPs。通过紫外可见光谱证实了AgNPs的成功合成，其在428nm处显示出表面等离子体共振峰。AgNPs的晶体尺寸为24.1nm。动态光散射分析表明，氧化银纳米颗粒的直径为179.3nm，表面电荷为-18mV。全面的体外药物遗传学特性表明，AgNPs具有显著的治疗潜力。通过测定对各种微生物菌株的最低抑菌浓度来评估AgNPs的抗菌性能。使用不同浓度的AgNPs对利什曼原虫前鞭毛体（IC：18.71μg/mL）、无鞭毛体（IC50：38.6μg/mL）和卤虫（IC：134.1μg/mL）进行剂量依赖性细胞毒性试验。本研究结果表明，AgNPs具有显著的抗氧化结果（DPPH：57.5%，TRP：55.4%，TAC：61%）以及对蛋白激酶（抑菌圈：17.11mm）和α-淀粉酶（25.3%）的酶抑制潜力。此外，还对巨噬细胞（IC：>395μg/mL）和人红细胞（IC：2124μg/mL）进行了生物相容性测试。本研究表明，藻类合成的AgNPs毒性较小，可用于多种生物应用，包括药物设计以及制药和生物医学行业。本研究提供了有价值的见解，并为AgNPs研究的进一步发展铺平了道路。
