Badeggi Umar M, Omoruyi Sylvester I, Ismail Enas, Africa Charlene, Botha Subelia, Hussein Ahmed A
Chemistry Department, Cape Peninsula University of Technology, Symphony Rd., Bellville 7535, South Africa.
Department of Restorative Dentistry, Faculty of Dentistry, University of the Western Cape, Cape Town 7505, South Africa.
Plants (Basel). 2022 Apr 11;11(8):1037. doi: 10.3390/plants11081037.
The reducing potential of plant extracts in the green synthesis of nanoparticles has been associated with their phytochemicals. Although pharmacologically inactive, a norlignan diglucoside "hypoxoside" (HP) occurs in large quantities in the extract of (HE). In this work, HP was isolated from HE where both were used in the biosynthesis of the corresponding silver nanoparticles (HP-AgNPs and HE-AgNPs). The AgNPs were fully characterized using various physicochemical techniques and their antimicrobial and anticancer properties were evaluated. Transmission electron microscopy (TEM) revealed sizes of 24.3 ± 4 nm for the HE-AgNPs and 3.9 ± 1.6 nm for the HP-AgNPs. The HE-AgNPs demonstrated enhanced anti-bactericidal effects on and with a minimum inhibitory concentration (MIC) value of 1.95 µg/mL, competing well with the standard drug. The cytotoxic activity showed that the HE-AgNPs reduced cell viability with an IC50 of 0.81 and 4.0 µg/mL, respectively, for the U87 and U251 cells, while the HP-AgNPs displayed 0.20 and 0.55 µg/mL for both cell lines, respectively. Furthermore, while the HE-AgNPs were selective to U87 alone, the HP-AgNPs were selective to both glioblastoma cells tested. The study demonstrated the ability of a single phytoconstituent (hypoxoside), not only as the chief bioreductant in the extract, but also as a standalone reducing and capping agent, producing ultra-small, spherical, and monodispersed AgNPs with enhanced biological properties.
植物提取物在纳米颗粒绿色合成中的还原潜力与其植物化学物质有关。尽管一种去甲木脂素二糖苷“氧杂环丁烷苷”（HP）在（植物提取物名称未给出）提取物中大量存在，却无药理活性。在本研究中，从该植物提取物中分离出HP，并分别将二者用于相应银纳米颗粒（HP-AgNPs和HE-AgNPs）的生物合成。使用多种物理化学技术对银纳米颗粒进行了全面表征，并评估了它们的抗菌和抗癌特性。透射电子显微镜（TEM）显示，HE-AgNPs的尺寸为24.3±4 nm，HP-AgNPs的尺寸为3.9±1.6 nm。HE-AgNPs对（未明确菌名）和（未明确菌名）表现出增强的杀菌作用，最低抑菌浓度（MIC）值为1.95 µg/mL，与标准药物相当。细胞毒性活性表明，对于U87和U251细胞，HE-AgNPs降低细胞活力的IC50分别为0.81和4.0 µg/mL，而HP-AgNPs对两种细胞系的IC50分别为0.20和0.55 µg/mL。此外，虽然HE-AgNPs仅对U87有选择性，但HP-AgNPs对所测试的两种胶质母细胞瘤细胞均具有选择性。该研究证明了单一植物成分（氧杂环丁烷苷）不仅作为提取物中的主要生物还原剂，还可作为独立的还原和封端剂，能够产生具有增强生物学特性的超小、球形且单分散的银纳米颗粒。
