Zhao Qinghua, Wang Haifeng, Yang Mingying, Yang Delin, Zuo Yigang, Wang Jiansong
Department of Gynaecology, The Second Affiliated Hospital of Kunming Medical University, Yunnan Institute of Urology, Kunming 650101, P.R. China.
Exp Ther Med. 2013 Mar;5(3):942-946. doi: 10.3892/etm.2013.892. Epub 2013 Jan 11.
Homo sapiens longevity assurance homolog 2 of yeast LAG1 (LASS2), a metastasis suppressor gene of human cancer, is the most abundantly expressed member of the ceramide synthase gene family. Expression of LASS2 has been reported in carcinomas of the prostate, liver and breast. However, there has been no report on the expression of LASS2 in human bladder cancer cell lines. In order to investigate the expression and potential role of this new tumor metastasis supressor gene in human bladder cancer, we compared the proliferation, metastasis and invasion among the BIU-87, T24, EJ and EJ-M3 human bladder cancer cell lines. The mRNA expression levels of the LASS2 gene were examined using real-time quantitative PCR (qPCR). The expression levels of LASS1 and LASS3 mRNA were used as references. The protein expression level of the LASS2 gene was detected using western blotting. The most aggressive of these four human cancer cell lines was observed to be EJ-M3. The expression of LASS2 mRNA was significantly correlated with diverse proliferation, metastasis and invasion. The expression levels of LASS1 and LASS3 mRNA were not correlated with these parameters. At the protein level, we observed that the more aggressive the cancer cell line, the lower the LASS2 protein expression level. Therefore, LASS2 expression may be correlated with the development and progression of human bladder cancer and may be a prognostic indicator for this cancer.
酵母LAG1的人类同源物长寿保证同源蛋白2（LASS2）是人类癌症的一种转移抑制基因，是神经酰胺合酶基因家族中表达最丰富的成员。已有报道称LASS2在前列腺癌、肝癌和乳腺癌中表达。然而，关于LASS2在人膀胱癌细胞系中的表达尚无报道。为了研究这个新的肿瘤转移抑制基因在人膀胱癌中的表达及潜在作用，我们比较了BIU - 87、T24、EJ和EJ - M3人膀胱癌细胞系之间的增殖、转移和侵袭情况。使用实时定量PCR（qPCR）检测LASS2基因的mRNA表达水平。以LASS1和LASS3 mRNA的表达水平作为对照。使用蛋白质印迹法检测LASS2基因的蛋白质表达水平。观察发现这四种人癌细胞系中侵袭性最强的是EJ - M3。LASS2 mRNA的表达与不同的增殖、转移和侵袭显著相关。LASS1和LASS3 mRNA的表达水平与这些参数无关。在蛋白质水平上，我们观察到癌细胞系的侵袭性越强，LASS2蛋白质表达水平越低。因此，LASS2的表达可能与人膀胱癌的发生发展相关，并且可能是该癌症的一个预后指标。
