在扩展固定边界中的电泳。
Electrophoresis in an expanded stationary boundary.
作者信息
Michov B M
机构信息
Department of Biochemistry, Academy of Medicine, Sofia.
出版信息
Electrophoresis. 1990 Apr;11(4):289-92. doi: 10.1002/elps.1150110403.
PMID:2340822
Abstract
A method of electrophoresis in an expanded stationary boundary is described, in which a pH gradient formed in a single buffer is used. The degrees of ionization and the concentrations of the electrolytes have values at which the polyions stack and resolve simultaneously in a polyacrylamide gel. To illustrate the method, the resolving of serum proteins in a pH gradient formed by Tris-glycinate buffer is considered.
摘要
描述了一种在扩展的固定边界中进行电泳的方法，其中使用在单一缓冲液中形成的pH梯度。电解质的电离度和浓度具有多离子在聚丙烯酰胺凝胶中同时堆积和分离的值。为了说明该方法，考虑了在Tris-甘氨酸缓冲液形成的pH梯度中血清蛋白的分离。
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