Manara G C, Soligo D, Lambertenghi-Deliliers G, Ferrari C, De Panfilis G
Department of Dermatology, University of Parma, Italy.
Dermatologica. 1990;180(3):141-5. doi: 10.1159/000248014.
An immunogold technique in scanning electron microscopy was applied to the detection of Langerhans cell surface CD1a antigen in a heterogeneous human epidermal cell suspension. Scanning electron microscopy investigations performed in the secondary electron imaging mode and in the backscattered electron imaging mode allowed to define Langerhans cell surface morphology and the labeling degree, respectively. In particular, the backscattered electron imaging mode of the scanning electron microscope appeared very useful in identifying colloidal gold particles and, as a result, made it possible to make total counts of the labeled surface antigenic sites. Finally, the combination of secondary electron and backscattered electron signals generated mixed images with the Langerhans cell surface morphology well recognizable together with distinct gold particles. The immunogold labeling in scanning electron microscopy here described represents a highly sensitive method and yields a new improvement in the study of Langerhans cells immunophenotype.
一种扫描电子显微镜免疫金技术被应用于检测异质性人表皮细胞悬液中的朗格汉斯细胞表面CD1a抗原。在二次电子成像模式和背散射电子成像模式下进行的扫描电子显微镜研究分别能够确定朗格汉斯细胞的表面形态和标记程度。特别是，扫描电子显微镜的背散射电子成像模式在识别胶体金颗粒方面显得非常有用，因此能够对标记的表面抗原位点进行总数统计。最后，二次电子信号和背散射电子信号的结合产生了混合图像，其中朗格汉斯细胞的表面形态清晰可辨，同时还有明显的金颗粒。本文所述的扫描电子显微镜免疫金标记是一种高度灵敏的方法，为朗格汉斯细胞免疫表型的研究带来了新的改进。
