de Harven E, Leung R, Christensen H
J Cell Biol. 1984 Jul;99(1 Pt 1):53-7. doi: 10.1083/jcb.99.1.53.
A method is described for the use of scanning electron microscopy on the surface of gold-labeled cells. It includes the use of 45- or 20-nm colloidal gold marker conjugated with Staphylococcal protein A. The marker is best recognized on the basis of its atomic number contrast by using the backscattered electron imaging mode of the scanning electron microscope. When the backscattered electron signal is mixed with the secondary electron signal, an optimum correlation between the distribution of the labeled sites and the cell surface structures is demonstrated. The method is illustrated by its application to the identification of human circulating granulocytes. Its good resolution, high contrast, and good labeling efficiency offers a promising approach to the specific localization of cell surface antigenic sites labeled with particles of colloidal gold.
本文描述了一种用于对金标记细胞表面进行扫描电子显微镜观察的方法。该方法包括使用与葡萄球菌蛋白A偶联的45纳米或20纳米胶体金标记物。通过扫描电子显微镜的背散射电子成像模式，基于其原子序数对比度能够很好地识别该标记物。当背散射电子信号与二次电子信号混合时，标记位点的分布与细胞表面结构之间呈现出最佳的相关性。通过将该方法应用于鉴定人类循环粒细胞来说明其用途。其良好的分辨率、高对比度和良好的标记效率为用胶体金颗粒标记的细胞表面抗原位点的特异性定位提供了一种有前景的方法。
