一种用于胶体金标记细胞表面扫描电子显微镜观察的新方法。
A novel approach for scanning electron microscopy of colloidal gold-labeled cell surfaces.
作者信息
de Harven E, Leung R, Christensen H
出版信息
J Cell Biol. 1984 Jul;99(1 Pt 1):53-7. doi: 10.1083/jcb.99.1.53.
Abstract
A method is described for the use of scanning electron microscopy on the surface of gold-labeled cells. It includes the use of 45- or 20-nm colloidal gold marker conjugated with Staphylococcal protein A. The marker is best recognized on the basis of its atomic number contrast by using the backscattered electron imaging mode of the scanning electron microscope. When the backscattered electron signal is mixed with the secondary electron signal, an optimum correlation between the distribution of the labeled sites and the cell surface structures is demonstrated. The method is illustrated by its application to the identification of human circulating granulocytes. Its good resolution, high contrast, and good labeling efficiency offers a promising approach to the specific localization of cell surface antigenic sites labeled with particles of colloidal gold.
摘要
本文描述了一种用于对金标记细胞表面进行扫描电子显微镜观察的方法。该方法包括使用与葡萄球菌蛋白A偶联的45纳米或20纳米胶体金标记物。通过扫描电子显微镜的背散射电子成像模式，基于其原子序数对比度能够很好地识别该标记物。当背散射电子信号与二次电子信号混合时，标记位点的分布与细胞表面结构之间呈现出最佳的相关性。通过将该方法应用于鉴定人类循环粒细胞来说明其用途。其良好的分辨率、高对比度和良好的标记效率为用胶体金颗粒标记的细胞表面抗原位点的特异性定位提供了一种有前景的方法。
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本文引用的文献
1
Studies on the fibrinogen, dextran and phytohemagglutinin methods of isolating leukocytes.关于纤维蛋白原、右旋糖酐和植物血凝素分离白细胞方法的研究。
Blood. 1956 May;11(5):436-54.
2
Cell surface distribution of fibronectin in cultures of fibroblasts and bladder derived epithelium: SEM-immunogold localization compared to immunoperoxidase and immunofluorescence.成纤维细胞和膀胱来源上皮细胞培养物中纤连蛋白的细胞表面分布：与免疫过氧化物酶和免疫荧光法相比的扫描电镜免疫金定位研究
J Microsc. 1981 Aug;123(Pt 2):227-36. doi: 10.1111/j.1365-2818.1981.tb01297.x.
3
A review of cell surface markers and labelling techniques for scanning electron microscopy.扫描电子显微镜细胞表面标志物及标记技术综述
Histochem J. 1980 May;12(3):273-315. doi: 10.1007/BF01006952.
4
Ultrastructural cytochemical localizations by back-scattered electron imaging of white blood cells.
J Histochem Cytochem. 1981 Sep;29(9):1071-9. doi: 10.1177/29.9.6945361.
5
The fine structure of normal lymphocyte subpopulations--a study with monoclonal antibodies and the immunogold technique.正常淋巴细胞亚群的精细结构——一项使用单克隆抗体和免疫金技术的研究
Clin Exp Immunol. 1982 Nov;50(2):416-25.
6
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Proc Natl Acad Sci U S A. 1973 Sep;70(9):2492-6. doi: 10.1073/pnas.70.9.2492.
7
Visualization of concanavalin A-binding sites with scanning electron microscopy.用扫描电子显微镜观察伴刀豆球蛋白A结合位点
J Cell Biol. 1974 Nov;63(2 Pt 1):699-707. doi: 10.1083/jcb.63.2.699.
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J Cell Biol. 1975 Jul;66(1):198-200. doi: 10.1083/jcb.66.1.198.
9
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J Exp Med. 1975 Feb 1;141(2):518-23. doi: 10.1084/jem.141.2.518.
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