Fell Robert A, Zee Kwan-Yat, Arora Manish
Discipline of Periodontics, Faculty of Dentistry, University of Sydney, Australia.
J Int Acad Periodontol. 2013 Jan;15(1):20-8.
To investigate the correlation between the gingival crevicular fluid (GCF) levels of IL-6 and TNF-alpha with the levels in serum in obese patients.
Twenty-six obese adults (BMI > or = 30, age 33-74) provided serum and GCF samples. Smokers and uncontrolled diabetics (HbA1c > 8%) were excluded. Serum and GCF samples were analysed for IL-6 and TNF-alpha using commercially available ELISA kits. Within each subject GCF was collected from two healthy sites (n = 26 subjects) and two gingivitis sites, defined by bleeding on probing (n = 22 subjects). The levels of IL-6 and TNF-alpha in the GCF were compared and correlated with the levels found in serum using Spearman's correlation analysis. A Bland-Altman analysis was used to determine the level of agreement between serum and GCF samples.
IL-6 was more frequently detected than TNF-alpha. This was consistent in serum (100% vs 64%) and GCF samples from healthy (73% vs 52%) and gingivitis (95% vs 36%) sites. There were no significant correlations between the TNF-alpha in serum and GCF samples from healthy (r = 0.27, p = 0.22) and gingivitis (r = -0.19, p = 0.40) sites. In contrast, positive correlations were found for IL-6 between serum and GCF samples from healthy (r = 0.48, p = 0.03) and gingivitis (r = 0.79, p = 0.0001) sites. The correlation and agreement was strongest for IL-6 between serum and gingivitis GCF samples.
The results of this pilot study suggest a lack of correlation and poor agreement between serum and GCF samples in obese subjects. Studies examining the link between periodontitis and obesity should consider collecting both serum and GCF.
研究肥胖患者龈沟液（GCF）中白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平与血清中相应水平的相关性。
26名肥胖成年人（体重指数[BMI]≥30，年龄33 - 74岁）提供了血清和GCF样本。吸烟者和未控制的糖尿病患者（糖化血红蛋白[HbA1c]＞8%）被排除。使用市售酶联免疫吸附测定（ELISA）试剂盒分析血清和GCF样本中的IL-6和TNF-α。在每个受试者中，从两个健康部位（n = 26名受试者）和两个探诊出血定义的牙龈炎部位（n = 22名受试者）采集GCF。使用Spearman相关分析比较GCF中IL-6和TNF-α的水平，并将其与血清中发现的水平进行相关性分析。采用Bland-Altman分析来确定血清和GCF样本之间的一致性水平。
IL-6比TNF-α更常被检测到。这在血清（100%对64%）以及来自健康部位（73%对52%）和牙龈炎部位（95%对36%）的GCF样本中都是一致的。血清和来自健康部位（r = 0.27，p = 0.22）以及牙龈炎部位（r = -0.19，p = 0.40）的GCF样本中的TNF-α之间没有显著相关性。相比之下，血清与来自健康部位（r = 0.48，p = 0.03）和牙龈炎部位（r = 0.79，p = 0.0001）的GCF样本中的IL-6之间发现了正相关性。血清和牙龈炎GCF样本中IL-6的相关性和一致性最强。
这项初步研究的结果表明，肥胖受试者的血清和GCF样本之间缺乏相关性且一致性较差。研究牙周炎与肥胖之间联系的研究应考虑同时采集血清和GCF。
