Gupta D, Zarzycki J, Rager K
Steroids. 1975 Jan;25(1):33-42. doi: 10.1016/s0039-128x(75)80005-5.
Plasma concentration of testosterone and dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one) were measured at frequent intervals (daily between day 20 and 40) in intact male rats from early pre-pubertal stage to full maturity. Effect of orchidectomy and experimental bilateral cryptorchidism on these two blood-born compounds at different stages of sexual maturation was also studied. A distinct spurt in testosterone level was noted on day 26, and in 3 days a leval of 2600pg/ml was reached, which was about 90% of the highest peak value (2940 pg/ml) seen at day 70. In the same animals dihydrotestosterone spurt was noted at the same time, but the peak value (163 pg/ml) was reached later (day 33). Within 4 days after orchidectomy plasma testosterone level showed no significant change in 20-day old animals, but dropped to 15% of the intact value in 40-day old ones, while remaining at a steady level of about 23% in older animals. Plasma dihydrotestosterone was undetectable after orchidectomy at each stage of sexual maturation. With bilateral cryptorchid animals plasma testosterone was higher than seen in the intact 20-day old control, but remained similar at other stages of sexual maturation. Plasma dihydrotesterone in the same animals was significantly less (p less 0.01) than those seen in the intact controls at all stages of sexual development except in 20-day old ones, where it was essentially similar.
在从青春期前早期到完全成熟的完整雄性大鼠中，频繁（在第20天至40天之间每天）测量睾酮和双氢睾酮（17β-羟基-5α-雄甾烷-3-酮）的血浆浓度。还研究了去势和实验性双侧隐睾对这两种血源性化合物在性成熟不同阶段的影响。在第26天观察到睾酮水平有明显的激增，3天内达到2600pg/ml的水平，这约为第70天所见最高峰值（2940pg/ml）的90%。在同一批动物中，双氢睾酮也在同一时间出现激增，但峰值（163pg/ml）出现较晚（第33天）。去势后4天内，20日龄动物的血浆睾酮水平无显著变化，但40日龄动物的血浆睾酮水平降至完整动物值的15%，而老年动物则保持在约23%的稳定水平。在性成熟的每个阶段，去势后血浆双氢睾酮均无法检测到。对于双侧隐睾动物，其血浆睾酮水平高于完整的20日龄对照动物，但在性成熟的其他阶段保持相似。除20日龄动物（其血浆双氢睾酮水平基本相似）外，同一批动物在性发育的所有阶段的血浆双氢睾酮水平均显著低于完整对照动物（p<0.01）。
