State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Anal Chem. 2013 Mar 19;85(6):3363-8. doi: 10.1021/ac400041t. Epub 2013 Mar 7.
In this work, we fabricate an oriented luminescent quantum dot (QD)-layered double hydroxide (LDH) nanocomposite material by the highly orderly and alternate assembly of trace CdTe QDs in dodecylbenzene sulfonate bilayer bunches on the organo-modified LDH exterior surfaces. Interestingly, the novel QD-LDH nanocomposites can remarkably amplify chemiluminescence (CL) of the luminol-H2O2 system, which is attributed to an inhibition of QD oxidation by H2O2, an increase in the radiative decay rate, and an inhibition in the nonradiative relaxation of QDs. In addition, a novel flow-through column-based CL resonance energy transfer is fabricated using luminol as energy donors and the solid luminescent QD-LDH nanocomposites as energy acceptors for signal amplification. The applicability of this flow-through column is evaluated by determining H2O2 using luminol-H2O2 CL system. The CL intensity exhibits a stable response to H2O2 over a concentration range from 0.5 to 60 μM with a detection limit as low as 0.3 μM. Finally, the proposed method has been successfully applied to detect H2O2 in snow samples, and the results agreed with those obtained by the standard spectrophotometric method. Our findings indicate that the new luminescent QD-LDH nanocomposite material would be used for high throughput screening of complex systems with different sized QDs.
在这项工作中，我们通过在有机改性层状双氢氧化物（LDH）外表面上的十二烷基苯磺酸盐双层束中痕量 CdTe QD 的高度有序和交替组装，制造了一种定向发光量子点（QD）-层状双氢氧化物（LDH）纳米复合材料。有趣的是，新型 QD-LDH 纳米复合材料可以显著放大鲁米诺-H2O2 体系的化学发光（CL），这归因于 H2O2 抑制了 QD 的氧化，增加了辐射衰减率，并抑制了 QD 的非辐射弛豫。此外，使用鲁米诺作为能量供体，将固态发光 QD-LDH 纳米复合材料作为能量受体，构建了一种新颖的基于流通柱的 CL 共振能量转移，用于信号放大。通过使用鲁米诺-H2O2 CL 系统来确定 H2O2，评估了这种流通柱的适用性。CL 强度在 0.5 至 60 μM 的浓度范围内对 H2O2 表现出稳定的响应，检测限低至 0.3 μM。最后，该方法已成功用于检测雪样中的 H2O2，结果与标准分光光度法一致。我们的研究结果表明，新型发光 QD-LDH 纳米复合材料将用于不同尺寸 QD 的复杂体系的高通量筛选。
