Sadamoto Hisayo, Muto Hideki
Laboratory of Functional Biology, Tokushima Bunri University, Sanuki, Kagawa, Japan.
Methods Mol Biol. 2013;977:229-41. doi: 10.1007/978-1-62703-284-1_18.
Fluorescence cross-correlation spectroscopy (FCCS) is an established spectroscopic method to observe the interaction between the different fluorescent molecules. Using FCCS, researchers can assess the interaction of target molecules in the aqueous condition, and can apply the technique in cultured cells. Here, we describe the method of FCCS to demonstrate direct observation of dimerization between transcription factors in a living cell.
荧光交叉相关光谱法（FCCS）是一种用于观察不同荧光分子间相互作用的成熟光谱方法。利用FCCS，研究人员能够在水性条件下评估靶分子的相互作用，并可将该技术应用于培养细胞。在此，我们描述FCCS方法，以展示对活细胞中转录因子二聚化的直接观察。
