Bagheri Yousef, Ali Ahsan Ausaf, You Mingxu
Department of Chemistry, University of Massachusetts, Amherst, MA, United States.
Front Chem. 2020 Dec 7;8:603259. doi: 10.3389/fchem.2020.603259. eCollection 2020.
Short-lived cell membrane complexes play a key role in regulating cell signaling and communication. Many of these complexes are formed based on low-affinity and transient interactions among various lipids and proteins. New techniques have emerged to study these previously overlooked membrane transient interactions. Exciting functions of these transient interactions have been discovered in cellular events such as immune signaling, host-pathogen interactions, and diseases such as cancer. In this review, we have summarized current experimental methods that allow us to detect and analyze short-lived cell membrane protein-protein, lipid-protein, and lipid-lipid interactions. These methods can provide useful information about the strengths, kinetics, and/or spatial patterns of membrane transient interactions. However, each method also has its own limitations. We hope this review can be used as a guideline to help the audience to choose proper approaches for studying membrane transient interactions in different membrane trafficking and cell signaling events.
短命的细胞膜复合物在调节细胞信号传导和通讯中起关键作用。这些复合物中的许多是基于各种脂质和蛋白质之间的低亲和力和瞬时相互作用形成的。已出现新技术来研究这些先前被忽视的膜瞬时相互作用。这些瞬时相互作用在诸如免疫信号传导、宿主-病原体相互作用以及癌症等疾病等细胞事件中已发现令人兴奋的功能。在本综述中，我们总结了当前可用于检测和分析短命的细胞膜蛋白-蛋白、脂质-蛋白和脂质-脂质相互作用的实验方法。这些方法可以提供有关膜瞬时相互作用的强度、动力学和/或空间模式的有用信息。然而，每种方法也有其自身的局限性。我们希望本综述可作为指南，帮助读者选择合适的方法来研究不同膜运输和细胞信号事件中的膜瞬时相互作用。
