Tiwari Manisha, Kinjo Masataka
Laboratory for Nano-Bio Probes, Quantitative Biology Center, OLABB, Osaka University, Suita, Japan.
Methods Mol Biol. 2015;1228:173-86. doi: 10.1007/978-1-4939-1680-1_14.
Fluorescence cross-correlation spectroscopy (FCCS) is a promising technique for observing and quantifying protein-protein interactions in vitro and in vivo. FCCS has emerged as a useful tool for obtaining parameters of the concentration of labeled particles, their molecular dynamics, as well as the size of their complexes. This chapter discusses aspects of preparing a biological system for FCCS experiments and suggests practical advice for performing FCCS in living cells. Moreover, we describe the method of FCCS to determine the dissociation constant of a transcription factor dimer in the living cell.
荧光交叉相关光谱技术（FCCS）是一种在体外和体内观察和定量蛋白质 - 蛋白质相互作用的有前景的技术。FCCS已成为一种有用的工具，可用于获取标记颗粒浓度、其分子动力学以及复合物大小等参数。本章讨论了为FCCS实验准备生物系统的各个方面，并给出了在活细胞中进行FCCS的实用建议。此外，我们描述了用FCCS方法测定活细胞中转录因子二聚体解离常数的方法。
