Department of Colorectal and Anal Surgery, The Third Affiliated Hospital of Jilin University, Changchun, Jilin, PR China.
Oncol Rep. 2013 May;29(5):1932-8. doi: 10.3892/or.2013.2300. Epub 2013 Feb 22.
Emerging evidence has demonstrated the altered expression of mRNAs in cancer development and progression. In this study, the precise role of miRNA-22 (miR-22) in colon cancer cells was investigated. Upon transfection with a miR-22 expression vector, the viability of HCT-116 human colon cancer cells was significantly reduced and tumor cell migration and invasion capacity were also suppressed. Computational in silico analysis predicted that T-cell lymphoma invasion and metastasis 1 (TIAM1) is a target gene of miR-22. This was confirmed by qRT-PCR and western blotting, which showed that miR-22 expression inhibited TIAM1 mRNA and protein expression, respectively. In addition, the expression of pro-invasive gene matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) and pro-angiogenic protein vascular endothelial growth factor (VEGF) were also reduced by miR-22 expression. Collectively, these data suggest that miR-22 may act as a tumor suppressor in colon cancer, most likely by targeting TIAM1 expression.
新兴证据表明，mRNA 的表达改变在癌症的发生和发展中起作用。在这项研究中，研究人员调查了 microRNA-22（miR-22）在结肠癌细胞中的精确作用。转染 miR-22 表达载体后，人结肠癌细胞 HCT-116 的活力明显降低，肿瘤细胞迁移和侵袭能力也受到抑制。计算生物信息学分析预测 T 细胞淋巴瘤侵袭和转移 1（TIAM1）是 miR-22 的一个靶基因。这通过 qRT-PCR 和 Western blot 得到了证实，结果显示 miR-22 的表达分别抑制了 TIAM1 mRNA 和蛋白的表达。此外，miR-22 的表达还降低了促侵袭基因基质金属蛋白酶 2 和 9（MMP-2 和 MMP-9）以及促血管生成蛋白血管内皮生长因子（VEGF）的表达。总之，这些数据表明 miR-22 可能在结肠癌中作为一种肿瘤抑制因子发挥作用，其作用机制可能是通过靶向 TIAM1 的表达。
