Oral Microbiology and Immunology, Institute of Oral Biology, Center of Dental Medicine, University of Zürich, Zürich, Switzerland.
J Periodontal Res. 2013 Dec;48(6):727-32. doi: 10.1111/jre.12061. Epub 2013 Feb 27.
Synergistetes is a novel bacterial phylum consisting of gram-negative anaerobes. Increasing lines of evidence demonstrate that this phylum is associated with periodontal diseases. This study aimed to compare the presence and levels of Synergistetes clusters A and B, in saliva of patients with chronic periodontitis (CP), generalized aggressive periodontitis (G-AgP) and non-periodontitis subjects, and investigate their correlation with clinical parameters.
Saliva was collected from patients with CP (n = 20), G-AgP (n = 21) and non-periodontitis subjects (n = 18). Full mouth clinical periodontal measurements were recorded. The numbers of Synergistetes cluster A and cluster B or the associated species Jonquetella anthropi were quantified by fluorescent in situ hybridization and microscopy.
Synergistetes cluster A bacteria were detected more frequently, and at higher numbers and proportions in the two periodontitis groups, than the non-periodontitis control group. The prevalence was 27.7% in the control group, 85% in CP and 86% in G-AgP. Compared to the control group, the numbers were significantly higher by 12.5-fold in CP and 26.5-fold in G-AgP, whereas the difference between the two forms of periodontitis was not statistically significant. Within the total bacterial population, the proportion of this cluster was increased in CP and G-AgP compared to the control group, with the difference between the two forms of periodontitis being also significant. There was a positive correlation between the levels of Synergistetes cluster A in saliva and all full mouth clinical periodontal parameters. Nevertheless, Synergistetes cluster B bacteria and J. anthropi species were detected infrequently and at low levels in all the three subject groups.
Synergistetes cluster A, but not cluster B, bacteria are found at higher prevalence, numbers and proportions in saliva from patients with periodontitis, than non-periodontitis subjects. These findings support the association of this cluster with periodontitis.
互养菌门是一个新的细菌门，由革兰氏阴性厌氧菌组成。越来越多的证据表明，该菌门与牙周病有关。本研究旨在比较慢性牙周炎（CP）、广泛性侵袭性牙周炎（G-AgP）和非牙周炎患者唾液中协同菌 A 群和 B 群的存在和水平，并探讨其与临床参数的相关性。
收集 CP 患者（n=20）、G-AgP 患者（n=21）和非牙周炎患者（n=18）的唾液。记录全口临床牙周测量值。采用荧光原位杂交和显微镜定量检测协同菌 A 群和 B 群或相关物种乔纳奎拉人（Jonquetella anthropi）的数量。
协同菌 A 群细菌在两个牙周炎组中的检出率、数量和比例均高于非牙周炎对照组。对照组的患病率为 27.7%，CP 组为 85%，G-AgP 组为 86%。与对照组相比，CP 组的数量增加了 12.5 倍，G-AgP 组增加了 26.5 倍，而两种形式的牙周炎之间的差异无统计学意义。在总细菌群体中，与对照组相比，CP 和 G-AgP 中该菌群的比例增加，两种形式的牙周炎之间的差异也具有统计学意义。唾液中协同菌 A 群的水平与全口临床牙周参数呈正相关。然而，在所有三组受试者中，协同菌 B 群细菌和 J. anthropi 种属的检出率较低，数量较少。
与非牙周炎患者相比，牙周炎患者唾液中协同菌 A 群细菌的检出率、数量和比例均较高。这些发现支持该菌群与牙周炎有关。
