Department of Dental Clinics, Division of Graduate Periodontics, School of Dentistry, Federal University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
J Periodontol. 2013 Sep;84(9):e9-e16. doi: 10.1902/jop.2013.120639. Epub 2013 Jan 17.
Suppuration (SUP) on probing may be an indication of active periodontal breakdown. The aim of the present study is to analyze which subgingival species are associated with SUP in patients with chronic (CP) and aggressive (AgP) periodontitis.
A total of 156 patients with CP and 66 with AgP were submitted to full-mouth periodontal examination and subgingival biofilm sampling (14 sites/patient). The counts of 44 bacterial species were determined by checkerboard. Comparisons between groups and sites were analyzed by the Mann-Whitney and Wilcoxon tests, respectively. Associations between frequency of SUP and bacterial species were analyzed by the Spearman correlation coefficient.
The prevalence of SUP in patients with CP was 24.4%, and in patients with AgP it was 30.3%, and the percentage of SUP sites in the groups was 5.72% ± 1.06% and 6.96% ± 1.70%, respectively (P >0.05). SUP sites from patients with CP had significantly higher counts of Veillonella parvula, Dialister pneumosintes, Tannerella forsythia, and Prevotella nigrescens than SUP sites from patients with AgP (P <0.005). Significant positive correlations between high frequency of SUP and high levels of Actinomyces spp, Streptococcus spp., members of the orange complex, Acinetobacter baumannii, and Pseudomonas aeruginosa were observed in patients with CP (P <0.05). In patients with AgP, Actinomyces oris, Propionibacterium acnes, P. aeruginosa, Staphylococcus aureus, and Streptococcus sanguinis were positively associated with SUP, whereas Prevotella intermedia presented a negative association with SUP (P <0.05).
SUP sites from patients with CP harbored significantly higher counts of several periodontal species than SUP sites from patients with AgP. Actinomyces spp., Streptococcus spp., members of the orange complex, T. forsythia, and certain non-oral pathogens were associated with a high number of sites with SUP.
探诊时有溢脓可能表明牙周破坏处于活动期。本研究旨在分析慢性（CP）和侵袭性（AgP）牙周炎患者中哪些龈下物种与探诊溢脓（SUP）相关。
共纳入 156 例 CP 患者和 66 例 AgP 患者，进行全口牙周检查和龈下生物膜采样（每位患者 14 个位点）。采用 checkerboard 法检测 44 种细菌的数量。组间和位点间比较分别采用 Mann-Whitney 和 Wilcoxon 检验。Spearman 相关系数分析 SUP 与细菌种类之间的相关性。
CP 患者的 SUP 患病率为 24.4%，AgP 患者为 30.3%，两组的 SUP 位点百分比分别为 5.72%±1.06%和 6.96%±1.70%（P＞0.05）。CP 患者的 SUP 位点中，韦荣球菌、小韦荣球菌、福赛斯坦纳菌和变黑普雷沃菌的检出率明显高于 AgP 患者（P＜0.005）。CP 患者中，SUP 高频与放线菌属、链球菌属、橙色复合群成员、鲍曼不动杆菌和铜绿假单胞菌高水平呈显著正相关（P＜0.05）。AgP 患者中，口腔放线菌、痤疮丙酸杆菌、铜绿假单胞菌、金黄色葡萄球菌和中间普雷沃菌与 SUP 呈正相关，而中间普雷沃菌与 SUP 呈负相关（P＜0.05）。
CP 患者的 SUP 位点中，几种牙周病物种的检出率明显高于 AgP 患者。放线菌属、链球菌属、橙色复合群成员、福赛斯坦纳菌和某些非口腔病原体与大量 SUP 位点相关。
