Department of Veterinary Medicine, National Chung-Hsing University, Taichung, Taiwan.
Food Chem. 2012 Oct 15;134(4):2169-75. doi: 10.1016/j.foodchem.2012.04.024. Epub 2012 Apr 17.
Carotenoids are effective antioxidants, which can quench singlet oxygen, suppress lipid peroxidation, and prevent oxidative damage. Both Pseudorabies virus (PRV) and human Herpes simplex virus (HSV) are DNA viruses, and their pathogenesis and immunobiology are similar. However, PRV does not infect humans. Therefore, PRV was used to infect murine macrophages (RAW264.7 cells), to mimic HSV-induced inflammation. Meanwhile, the influence of β-carotene on PRV-induced inflammation was also investigated. Results indicated that β-carotene inhibited (p<0.05) NO, IL-1β, IL-6, and MCP-1 production in PRV-infected RAW264.7 cells. β-Carotene also suppressed (p<0.05) NF-κB (p50 and p65), phosphorylation of extracellular-signal-related kinase (ERK), p38, and c-Jun N-terminal kinase (JNK) expression. It could be concluded that the anti-inflammatory effect of β-carotene is mainly through a suppression of cytokine expression in PRV-induced inflammation, which results from NF-κB inactivation. β-Carotene can be considered a potential anti-inflammatory agent for DNA-virus infection.
类胡萝卜素是有效的抗氧化剂，可以淬灭单线态氧，抑制脂质过氧化，防止氧化损伤。猪伪狂犬病病毒（PRV）和人单纯疱疹病毒（HSV）都是 DNA 病毒，它们的发病机制和免疫生物学相似。然而，PRV 并不感染人类。因此，使用 PRV 感染鼠巨噬细胞（RAW264.7 细胞），模拟 HSV 诱导的炎症。同时，还研究了 β-胡萝卜素对 PRV 诱导的炎症的影响。结果表明，β-胡萝卜素抑制了 PRV 感染的 RAW264.7 细胞中 NO、IL-1β、IL-6 和 MCP-1 的产生（p<0.05）。β-胡萝卜素还抑制了 NF-κB（p50 和 p65）、细胞外信号调节激酶（ERK）、p38 和 c-Jun N-末端激酶（JNK）的磷酸化表达（p<0.05）。可以得出结论，β-胡萝卜素的抗炎作用主要是通过抑制 PRV 诱导的炎症中的细胞因子表达来实现的，这是由于 NF-κB 失活所致。β-胡萝卜素可以被认为是一种有潜力的针对 DNA 病毒感染的抗炎剂。
