vanderSpek J C, Offner G D, Troxler R F, Oppenheim F G
Department of Biochemistry, Boston University School of Medicine, MA 02118.
Arch Oral Biol. 1990;35(2):137-43. doi: 10.1016/0003-9969(90)90175-a.
Histatins are a group of histidine-rich polypeptides found in human parotid and submandibular gland secretions. These polypeptides are microbiocidal, possibly involved in maintaining the acquired enamel pellicle, and enhance the glycolytic activity of certain oral micro-organisms. Histatins 1, 3 and 5 are homologous proteins with 38, 32 and 24 amino acid residues, respectively; the cDNAs coding for histatins 1 and 3 have now been isolated and sequenced. The cDNA sequences were highly homologous but contained differences throughout their length, indicating that they arise from different genes that may be derived from a common ancestral gene. Northern blots were hybridized to a series of oligonucleotide probes, designed on the basis of histatin cDNA sequences, and these positively identified mRNAs for histatins 1 and 3. In addition, there was a third mRNA, which hybridized to several histatin oligonucleotide probes, suggesting that histatin 5 might be derived from a distinct mRNA and not by proteolytic processing of histatin 3. A Northern blot of macaque parotid gland total RNA also showed three histatin mRNAs, indicating that similar histatins exist in a non-human primate.
富组蛋白是在人类腮腺和颌下腺分泌物中发现的一组富含组氨酸的多肽。这些多肽具有杀菌作用，可能参与维持获得性釉质薄膜，并增强某些口腔微生物的糖酵解活性。富组蛋白1、3和5是分别具有38、32和24个氨基酸残基的同源蛋白；编码富组蛋白1和3的cDNA现已被分离和测序。cDNA序列高度同源，但全长存在差异，这表明它们来自不同的基因，这些基因可能源自一个共同的祖先基因。用一系列基于富组蛋白cDNA序列设计的寡核苷酸探针与Northern印迹杂交，这些探针阳性鉴定出了富组蛋白1和3的mRNA。此外，还有第三种mRNA，它与几种富组蛋白寡核苷酸探针杂交，这表明富组蛋白5可能源自一种独特的mRNA，而不是通过富组蛋白3的蛋白水解加工产生。猕猴腮腺腺泡细胞总RNA的Northern印迹也显示出三种富组蛋白mRNA，表明在非人类灵长类动物中存在类似的富组蛋白。
