Frings-Meuthen P, Boehme G, Liphardt A-M, Baecker N, Heer M, Rittweger J
German Aerospace Center (DLR), Institute of Aerospace Medicine, Linder Hoehe, 51147 Cologne, Germany.
J Musculoskelet Neuronal Interact. 2013 Mar;13(1):45-52.
Wnt signaling pathway may be crucial in the pathogenesis of disuse-induced bone loss. Sclerostin and DKK1, antagonists of the Wnt signaling pathway, were assessed during immobilization by bed rest in young, healthy people.
Two bed rest studies were conducted at the German Aerospace Center in Cologne. 14 days of 6° head-down-tilt bed rest were applied to eight healthy young male test subjects in study 1 and 21 days of head-down-tilt bed rest to seven healthy male subjects in study 2.
Sclerostin levels increased in both studies during bed rest (study 1, 0.64±0.05 ng/ml to 0.69±0.04 ng/ml, P=0.014; study 2, 0.42±0.04 ng/ml to 0.47±0.04 ng/ml, P=0.008) and they declined at the end of the 14- and 21-day bed rest periods. DKK1 decreased during the bed rest period in study 1 (P<0.001) but increased during bed rest in study 2 (P=0.006). As expected, bone formation marker PINP decreased (study 1, P=0.013; study 2, P<0.001) and bone resorption marker NTX increased during bed rest (P<0.001).
Data suggest that the Wnt signaling pathway is involved in disuse-induced bone loss in young, healthy humans.
Wnt信号通路可能在废用性骨质流失的发病机制中起关键作用。在年轻健康人群卧床休息导致肢体固定期间，对Wnt信号通路的拮抗剂硬化蛋白和DKK1进行了评估。
在科隆的德国航空航天中心进行了两项卧床休息研究。研究1中，8名健康年轻男性受试者接受了14天6°头低位卧床休息；研究2中，7名健康男性受试者接受了21天头低位卧床休息。
两项研究中，卧床休息期间硬化蛋白水平均升高（研究1，从0.64±0.05 ng/ml升至0.69±0.04 ng/ml，P = 0.014；研究2，从0.42±0.04 ng/ml升至0.47±0.04 ng/ml，P = 0.008），且在14天和21天卧床休息期结束时下降。研究1中，卧床休息期间DKK1降低（P<0.001），而研究2中卧床休息期间DKK1升高（P = 0.006）。正如预期的那样，卧床休息期间骨形成标志物PINP降低（研究1，P = 0.0 — 13；研究2，P<0.001），骨吸收标志物NTX升高（P<0.001）。
数据表明，Wnt信号通路参与了年轻健康人群的废用性骨质流失。
