School of Biomedical Sciences, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University Bentley, Perth, Western Australia, Australia, 6102.
Folia Microbiol (Praha). 2013 Nov;58(6):469-74. doi: 10.1007/s12223-013-0232-z. Epub 2013 Feb 28.
The aim of this investigation was to determine the persistence of biofilm-associated antibiotic resistance developed by methicillin-sensitive Staphylococcus aureus (MSSA), of different capsular types, during biofilm formation. Because of superiority of the tissue culture plate (TCP) over the Congo Red Agar (CRA) method for measuring biofilm formation, it was used to determine the persistence of the antibiotic resistance developed by the isolates in biofilms. The antibiotic resistance was found to persist for 3-4 wk post-propagation as planktonic subcultures. Interestingly, some strains even developed resistance to vancomycin and/or teicoplanin. However, no association of either biofilm formation or persistent antibiotic resistance with the major capsular phenotype was observed. These observations highlight the potential significance of (a) determining the antibiograms of S. aureus subcultured from biofilms developed in vitro using the TCP method as well as from planktonic cultures for formulation of an optimal therapeutic strategy, and (b) continuing to identify predominant non-capsular antigens contributing to biofilm formation, regardless of the capsular phenotype for the development of an effective potentially broad-spectrum vaccine for prevention of bovine mastitis caused by S. aureus.
本研究旨在确定不同荚膜型耐甲氧西林金黄色葡萄球菌（MSSA）在生物膜形成过程中产生的生物膜相关抗生素耐药性的持久性。由于组织培养板（TCP）在测量生物膜形成方面优于刚果红琼脂（CRA）方法，因此该方法用于确定分离物在生物膜中产生的抗生素耐药性的持久性。研究发现，作为浮游亚培养物，抗生素耐药性在传播后 3-4 周内持续存在。有趣的是，一些菌株甚至对万古霉素和/或替考拉宁产生了耐药性。然而，没有观察到生物膜形成或持续抗生素耐药性与主要荚膜表型之间的任何关联。这些观察结果强调了以下方面的重要意义：（a）使用 TCP 方法从体外形成的生物膜中以及从浮游培养物中对金黄色葡萄球菌进行亚培养，确定抗生素图谱，以制定最佳治疗策略；（b）继续鉴定主要的非荚膜抗原，有助于生物膜形成，而不管开发有效、潜在广谱疫苗以预防金黄色葡萄球菌引起的牛乳腺炎的荚膜表型如何。
