Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry, India.
Bioprocess Biosyst Eng. 2013 Aug;36(8):1131-9. doi: 10.1007/s00449-012-0867-1. Epub 2013 Feb 28.
Green synthesis of selenium nanoparticles (SeNPs) was achieved by a simple biological procedure using the reducing power of fenugreek seed extract. This method is capable of producing SeNPs in a size range of about 50-150 nm, under ambient conditions. The synthesized nanoparticles can be separated easily from the aqueous sols by a high-speed centrifuge. These selenium nanoparticles were characterized by UV-Vis spectroscopy, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and elemental analysis by X-ray fluorescence spectrometer (XRF). Nanocrystalline SeNPs were obtained without post-annealing treatment. FTIR spectrum confirms the presence of various functional groups in the plant extract, which may possibly influence the reduction process and stabilization of nanoparticles. The cytotoxicity of SeNPs was assayed against human breast-cancer cells (MCF-7). It was found that SeNPs are able to inhibit the cell growth by dose-dependent manner. In addition, combination of SeNPs and doxorubicin shows better anticancer effect than individual treatments.
采用简单的生物法，利用葫芦巴种子提取物的还原能力，实现了硒纳米粒子（SeNPs）的绿色合成。在环境条件下，该方法能够生产出尺寸约为 50-150nm 的 SeNPs。合成的纳米粒子可以通过高速离心机很容易地从水溶胶中分离出来。通过紫外可见光谱、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）和 X 射线荧光能谱仪（XRF）对纳米硒进行了表征。无需进行后退火处理即可获得纳米晶硒。FTIR 谱图证实了植物提取物中存在各种官能团，这些官能团可能会影响还原过程和纳米粒子的稳定性。用硒纳米粒子对人乳腺癌细胞（MCF-7）进行了细胞毒性测定。结果发现，硒纳米粒子能够以剂量依赖的方式抑制细胞生长。此外，硒纳米粒子与阿霉素联合治疗的抗癌效果优于单独治疗。
