Abou Zeid Mayada A M, Okasha Lamiaa, Hegazy Yamen, Abdelmegeid Mohamed
Bacteriology , Kafrelsheikh Regional Laboratory, Animal Health Research Institute, Agricultural Research Center (ARC), Giza, Egypt.
Department of Animal Medicine, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.
Open Vet J. 2025 Feb;15(2):835-846. doi: 10.5455/OVJ.2025.v15.i2.33. Epub 2025 Feb 28.
Bovine subclinical mastitis is a significant disease that significantly impacts the health of dairy cows and subsequently affects milk production. Among the various causes of subclinical mastitis, bacterial infections, particularly play a crucial role.
The aim of this investigation was to isolate and identify strains from cows with subclinical mastitis, assess their antimicrobial susceptibility, virulence genes, and investigate the potential antibacterial activity of ethanolic extract and Selenium (Se) nanoparticles against both somatic cell count (SCC) and count in subclinical mastitic cows.
We collected 110 milk samples from dairy cows with subclinical mastitis in the Kafrelsheikh governorate before treatment with intramammary antibiotics. Samples were identified using pH detection, SCC at ≤200,000 cells/ ml, and California Mastitis Test.
The effect of ethanolic extract, Se nanoparticles, and Ciprofloxacin antibiotic in reducing SCC and counts was significant. Selenium nanoparticles and Ciprofloxacin antibiotics showed the highest reduction in the count, comparable to ethanolic extract, while ciprofloxacin followed by ethanolic extract, and Selenium nanoparticles were effective against somatic cell count.
This study emphasizes the widespread occurrence of in subclinical bovine mastitis and highlights the potential of ethanolic extract and Se nanoparticles as promising treatment options. Selenium nanoparticles demonstrated notable antibacterial effects against and contributed to a reduction in somatic cell counts. Considering these findings, we propose additional studies on the potential of Selenium nanoparticles as antibacterial agents, as they demonstrate effectiveness in preventing and managing subclinical mastitis.
牛亚临床型乳腺炎是一种严重疾病，对奶牛健康有重大影响，进而影响牛奶产量。在亚临床型乳腺炎的各种病因中，细菌感染尤其是[未提及的细菌]起着关键作用。
本研究旨在从患有亚临床型乳腺炎的奶牛中分离和鉴定[未提及的细菌]菌株，评估其抗菌药敏性、毒力基因，并研究[未提及的植物]乙醇提取物和硒（Se）纳米颗粒对亚临床型乳腺炎奶牛的体细胞计数（SCC）和[未提及的细菌]计数的潜在抗菌活性。
在使用乳房内抗生素治疗前，我们从卡夫勒谢赫省患有亚临床型乳腺炎的奶牛中收集了110份牛奶样本。通过pH检测、体细胞计数≤200,000个细胞/毫升以及加利福尼亚乳腺炎检测来鉴定样本。
[未提及的植物]乙醇提取物、硒纳米颗粒和环丙沙星抗生素在降低体细胞计数和[未提及的细菌]计数方面效果显著。硒纳米颗粒和环丙沙星抗生素在降低[未提及的细菌]计数方面表现出最高降幅，与[未提及的植物]乙醇提取物相当，而环丙沙星其次是[未提及的植物]乙醇提取物，硒纳米颗粒对体细胞计数有效。
本研究强调了[未提及的细菌]在亚临床型牛乳腺炎中广泛存在，并突出了[未提及的植物]乙醇提取物和硒纳米颗粒作为有前景的治疗选择的潜力。硒纳米颗粒对[未提及的细菌]显示出显著的抗菌作用，并有助于降低体细胞计数。考虑到这些发现，我们建议对硒纳米颗粒作为抗菌剂的潜力进行更多研究，因为它们在预防和管理[未提及的细菌]亚临床型乳腺炎方面显示出有效性。
