in subclinical bovine mastitis: Prevalence and innovative treatment with and Selenium nanoparticles.

BACKGROUND

Bovine subclinical mastitis is a significant disease that significantly impacts the health of dairy cows and subsequently affects milk production. Among the various causes of subclinical mastitis, bacterial infections, particularly play a crucial role.

AIM

The aim of this investigation was to isolate and identify strains from cows with subclinical mastitis, assess their antimicrobial susceptibility, virulence genes, and investigate the potential antibacterial activity of ethanolic extract and Selenium (Se) nanoparticles against both somatic cell count (SCC) and count in subclinical mastitic cows.

METHODS

We collected 110 milk samples from dairy cows with subclinical mastitis in the Kafrelsheikh governorate before treatment with intramammary antibiotics. Samples were identified using pH detection, SCC at ≤200,000 cells/ ml, and California Mastitis Test.

RESULTS

The effect of ethanolic extract, Se nanoparticles, and Ciprofloxacin antibiotic in reducing SCC and counts was significant. Selenium nanoparticles and Ciprofloxacin antibiotics showed the highest reduction in the count, comparable to ethanolic extract, while ciprofloxacin followed by ethanolic extract, and Selenium nanoparticles were effective against somatic cell count.

CONCLUSION

This study emphasizes the widespread occurrence of in subclinical bovine mastitis and highlights the potential of ethanolic extract and Se nanoparticles as promising treatment options. Selenium nanoparticles demonstrated notable antibacterial effects against and contributed to a reduction in somatic cell counts. Considering these findings, we propose additional studies on the potential of Selenium nanoparticles as antibacterial agents, as they demonstrate effectiveness in preventing and managing subclinical mastitis.