解淀粉芽胞杆菌 HBB-247 产生的细菌素样物质的分离与特性研究。
Isolation and Characterization of a Bacteriocin-Like Substance Produced by Geobacillus toebii Strain HBB-247.
机构信息
Faculty of Arts and Science, Biology Department, Adnan Menderes University, 09010 Aydin, Turkey.
出版信息
Indian J Microbiol. 2012 Mar;52(1):104-8. doi: 10.1007/s12088-011-0227-x. Epub 2011 Sep 14.
Abstract
A total of 201 thermophilic bacteria isolated from various thermal spring, mud and soil were tested for their antibacterial activity. Among the mostly active isolates, Geobacillus toebii HBB-247 was further examined. Bacteriocin-like inhibitory substance (BLIS) produced by strain HBB-247 was found to be stable up to 60°C, sensitive to proteolytic enzymes and effective against Enterococcus faecalis, Listeria sp., E. avium, Clostridium pasteurianum, Cellulomonas fimi and some thermophilic strains isolated and identified in this study. As a result of Tricine-SDS-PAGE molecular weight of BLIS was estimated about 38 kDa. Production studies showed that G. toebii HBB-247 starts to produce antibacterial substance at early logarithmic phase of growth and maximum production was detected at the end of the logarithmic phase.
摘要
从各种温泉、泥和土壤中分离出的 201 株嗜热菌被测试其抗菌活性。在大多数活性分离物中，进一步研究了 Geobacillus toebii HBB-247。发现菌株 HBB-247 产生的细菌素样抑制物质 (BLIS) 在高达 60°C 时稳定，对蛋白酶敏感，对粪肠球菌、李斯特菌、鸟肠球菌、丙酮丁醇梭菌、纤维单胞菌和本研究中分离和鉴定的一些嗜热菌株有效。由于 Tricine-SDS-PAGE，BLIS 的分子量约为 38 kDa。生产研究表明，G. toebii HBB-247 在对数生长早期开始产生抗菌物质，在对数生长末期检测到最大产量。
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