Production and characterization of a new antibacterial peptide obtained from SAT4.

A novel thermophilic bacterial strain of the genus was isolated from Thar Dessert Pakistan. This strain showed significant antibacterial activity against , , osa. The strain coded as 'SAT4' resembled with in the morphological, biochemical and molecular tests. The production of antibacterial metabolites by SAT4 was optimized. These active metabolites were precipitated by 50% ammonium sulphate and purified through sephadex G-75 gel permeation chromatography and reverse phase HPLC. The molecular weight of 37 kDa was examined by SDS-PAGE. The structural elucidation of the purified product was studied by FTIR, H and C NMR. The X-ray diffractions study showed that the crystals belonged to the primitive orthorhombic lattice ( = 12.137,  = 13.421,  = 14.097 Å) and 3D structure (proposed name: Aeritracin) was determined. This new peptide antibacterial molecule can get a position in pharmaceutical and biotechnological industrial research.