Sthitmatee Nattawooti, Yano Terdsak, Lampang Kannikar Na, Suphavilai Chaisuree, Kataoka Yasushi, Sawada Takuo
Department of Veterinary Bioscience and Veterinary Public Health, Faculty of Veterinary Medicine,Chiang Mai University, Chiang Mai 50100, Thailand.
J Vet Med Sci. 2013 Jul 31;75(7):923-8. doi: 10.1292/jvms.12-0475. Epub 2013 Mar 1.
The aim of this study was to show that a 39-kDa protein or OmpH of Pasteurella multocida strain P-1059 is essential for cross protection. Strain PBA322, a thinly capsulated strain of P. multocida strain P-1059, was used as a live vaccine in chickens. Strain PBA322 is a thinly capsulated strain in comparison with the parental strain P-1059. Chickens were vaccinated by single injection and then challenge-exposed with strains P-1059 or X-73 at two weeks post vaccination. Moreover, immune responses were also evaluated for both humoral and cellular immune response by ELISA and lymphocyte proliferation assay, respectively. The results showed that the live vaccine induced efficient immunity to protect chickens from challenge-exposure to the parent strain, but that the heterologous protection was poor. We concluded that the 39-kDa protein is essential for cross protection.
