Jarvis A W, Lawrence R C, Pritchard G G
J Gen Microbiol. 1975 Jan;86(1):75-87. doi: 10.1099/00221287-86-1-75.
The production of enterotoxins, lipase and total extracellular protein by four strains of Staphylococcus aureus grown in batch culture at a controlled pH of 6.5 in a completely defined medium was markedly reduced by glucose or glycerol constantly maintained at 0.I M. A concomitant increase in the production of deoxyribonuclease, up to 13-fold, showed however that not all extracellular proteins are under the same control mechanism. The presence of glucose and glycerol in the medium also resulted in a rapid increase in the specific growth rate. However, growth of S. aureus s6 in Mgilimited continuous culture showed that glucose repression of enterotoxin B when the growth rate was held constant was more than twice that in batch culture. Therefore glucose repression can occur independently of an increase in growth rate. The specific rate of production of enterotoxin B, lipase, deoxyribonuclease, beta-haemolysin and total extracellular protein by S. aureus s6 increased as the growth rate increased from 0.07 to 0.24 h-1. Non-replicating cells grown in the absence of glucose produced considerable amounts of enterotoxin, and production was not repressed by the presence of glucose in the resuspension medium. In contrast, no enterotoxin B or C was obtained from nonreplicating cells grown in the presence of glucose. Chloramphenicol completely inhibited enterotoxin production by non-replicating cells, indicating that synthesis of new protein was required.
在完全限定培养基中，于pH 6.5的条件下进行分批培养时，四株金黄色葡萄球菌产生的肠毒素、脂肪酶和总细胞外蛋白的量，会因持续维持在0.1 M的葡萄糖或甘油而显著减少。然而，脱氧核糖核酸酶的产量随之增加，最高可达13倍，这表明并非所有细胞外蛋白都受相同的调控机制控制。培养基中葡萄糖和甘油的存在还导致比生长速率迅速增加。不过，在Mg有限的连续培养中，金黄色葡萄球菌s6的生长表明，当生长速率保持恒定时，葡萄糖对肠毒素B的阻遏作用是分批培养中的两倍多。因此，葡萄糖阻遏可独立于生长速率的增加而发生。随着金黄色葡萄球菌s6的生长速率从0.07 h-1增加到0.24 h-1，其肠毒素B、脂肪酶、脱氧核糖核酸酶、β-溶血素和总细胞外蛋白的比产生速率也随之增加。在无葡萄糖条件下生长的非复制细胞会产生大量肠毒素，且重悬培养基中葡萄糖的存在不会抑制其产生。相比之下，在有葡萄糖条件下生长的非复制细胞未获得肠毒素B或C。氯霉素完全抑制非复制细胞产生肠毒素，这表明需要合成新的蛋白质。
